NADPH oxidase participates in the insect-resistant defense response of Arabidopsis thaliana induced by 1-pentene-3-one
As a volatile gas,ethyl vinyl ketone(1-penten-3-one)can trigger early signal generation and boost the insect resistance of Arabidopsis thaliana mesophyll cells.To investigate the role of plasma membrane NADPH oxidase in insect resistance in Arabidopsis induced by 1-penten-3-one,biological tests and real-time quantitative PCR were performed in WT Arabidopsis and two mutants of NADPH oxidase in plasma mem-brane(Atrbohd and Atrbohf).The results showed that fumigation with 1-pentene-3-one could stimulate the expression of NADPH oxidase gene in Arabidopsis,and NADPH oxidase may enhance Arabidopsis's capacity to resist insect pests by affecting the eating habits of Plutella xylostella.Additionally,NADPH oxidase contrib-uted to the expression of the defense genes Thi2.1,VSP1,PDF1.2,and VSP2 in Arabidopsis,which are stimu-lated by 1-pentene-3-one fumigation.This study aims to provide theoretical support for the molecular mech-anism of NADPH oxidase in increasing insect resistance in Arabidopsis induced by 1-pentene-3-one.
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