Effects of NaCl concentrations on nitrogen uptake and utilization of barley under low nitrogen conditions
To investigate the nitrogen absorption and utilization by salt-tolerant barley germplasm under the salt-stress conditions,the relevant traits under the hydroponic conditions were analyzed using a newly derived line DH20 seedlings from the barley parental variety'Hua30'by the combination of mutagenesis and microspore culture and the variety'Hua30'(parental control)as the experimental materials.Results showed that the seedling length,above-ground dry matter weight,nitrogen accumulation,stomatal con-ductance,and chlorophyll content of the new line DH20 and the control'Hua30'were reduced under the low nitrogen+zero salt treatment,but no significant differences were detected.Under medium salt(150 mmol·L-1 NaCl)conditions,the effects of low nitrogen treatment on the seedling length,above-ground dry matter weight,nitrogen accumulation,nitrogen uptake efficiency,nitrogen utilization rate,net photosyn-thetic rate,stomatal conductance,and Fv'/Fm'values of DH20 were significantly lower than those of'Hua30'.Under low nitrogen+high salt treatment,the seedling length,dry matter weight,nitrogen accumu-lation,nitrogen uptake efficiency of DH20 and'Hua30'were very low,but had no significant differences.Further analyses of the key nitrogen metabolism-associated genes(HvNR1,HvNRT3.1 and HvNRT3.3)in barley showed their significantly increased expression in DH20 under the low nitrogen+medium salt treat-ment,suggesting efficient absorption and utilization of nitrogen.These results indicated that the new salt-tolerant barley line DH20 had a potential for efficient absorption and using nitrogen under the condi-tion of medium salt stresses,and could be used as an important genetic resource(or future variety)in bar-ley breeding for efficient nitrogen utilization.
barleynew linesalt tolerancelow nitrogennitrogen uptake and utilization
NETL
NSTL
万方数据
