Identification of the NtZIP gene family and expression pattern analysis under cadmium stress in tobacco
The plant zinc-iron transporter protein,ZIP.is mainly involved in regulating the dynamic balance of essential metal elements,as well as participating in the absorption and transport of heavy metals such as cadmium(Cd).In this study,a total of 33 members of the NtZIP gene family were identified from the ge-nome of common tobacco(Nicotiana tabacum)based on a bioinformatics approach,of which 11 mem-bers were localized on seven chromosomes.Subcellular localization prediction shows that 26 NtZIP pro-teins were localized to the cell membrane and seven NtZIP proteins to the vacuole membrane.The 33 NtZIP members were classified into four subfamilies by the neighbour-joining method.Promoter analysis shows that the promoter regions of the NtZIP genes all contain abiotic stress and hormone-related cis-act-ing elements.Under Cd stress,quantitative real-time PCR results show that nine NtZIP genes are signifi-cantly responded to Cd in tobacco roots,which is basically consistent with the transcriptome results,but with the change of Cd concentration,NtZIP family members were differentially expressed,and it was hy-pothesized that NtZIP is involved in Cd uptake and translocation in tobacco.
Nicotiana tabacumZIP proteingene family analysiscadmium stress
NETL
NSTL
万方数据
