Expression and functional analysis of three sucrose synthase genes highly expressed in pod coats and seed coats of soybean
Sucrose is the main transport form of photosynthetic product in higher plants.Sucrose synthase(SUS)is a glycosyl transferase enzyme that plays a key role in sugar metabolism,primarily in sink tissues,therefore affects crop yield and quality.RNA-Seq data in our previous study showed that the soybean SUS5 subfamily genes,GmSUS5a,GmSUS5b and GmSUS5c were highly expressed in pods and seeds.However,their spatio-temporal expression patterns and functions have yet to be analyzed.Bioinformatics analysis showed that the GmSUS5s proteins share 82.0％and greater sequence similarity,encoding 840-845 ami-no acids and localized in cytoplasm.RT-qPCR analysis of pod coats and seeds at different developmental stages showed that the expression of the GmSUS5 genes reached at the highest level in pods and seed coats at seed filling stage,seven weeks after flowering,and the expression level of GmSUS5a was the highest.The single,double and triple mutants of GmSUS5 genes obtained by CRISPR/Cas9 gene editing showed prolonged flowering period and inefficiency in pod-setting,which may be due to the insufficient sugar supply to pods and embryos.This study confirmed that GmSUS5s are involved in seed sucrose sup-ply,thereby affecting soybean seed development.This study lays a foundation for further study of the function and mechanism of soybean sucrose synthases genes GmSUS5s.
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