64份醋栗番茄核心种质萌发期耐盐碱评价
Evaluation of saline-alkali tolerance of 64 core germplasms of Solanum pimpinellifolium at germination stage
孙明明 1郭冉博 1李鑫 2刘磊 2朱璨 2黄泽军 2王孝宣 2国艳梅 2杜永臣 2朱文莹 1李君明2
作者信息
- 1. 青岛农业大学园艺学院,山东青岛 266109
- 2. 中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京 100081
- 折叠
摘要
土壤盐碱化严重影响作物生产.野生种在适应外界环境的过程中,演化出较好的应对非生物胁迫的能力.鉴于当前在耐盐碱资源挖掘方面存在的不足,本研究对收集的野生醋栗番茄核心种质在Na2CO3胁迫条件下的萌发特性进行了评价.结果表明:Na2CO3溶液对番茄种子的萌发具有明显的抑制作用,但不同资源对Na2CO3胁迫的耐受性存在显著差异.在60 mmol·L-1的高Na2CO3浓度条件下,综合相对发芽势、相对发芽率与相对发芽指数,共鉴定出P8、P9、P12、P13、P25、P30、P40、P50、P51、P53、P56、P61、P62和P63等14份耐盐碱材料与22份盐碱敏感材料,耐盐碱材料相对发芽率与相对发芽势可达85％以上,其中P51与P61相对发芽率与相对发芽势达到100％,具有极强的耐盐碱能力.通过全基因组数据及已报道的来自醋栗番茄的耐盐碱SlScaBP8基因变异分析,推测这些资源的耐盐碱性状由不同基因控制,一些资源包含耐盐碱新位点,且耐盐和耐碱可能存在不同的调控机制.研究结果为利用野生资源进行耐盐碱遗传改良和机制解析提供了新依据.
Abstract
Soil salinization seriously affects crop production.Wild species have evolved a better ability to cope with abiotic stress in the process of adapting to the external environment.In view of the current defi-ciencies in the excavation of saline-alkali tolerant resources,the germination characteristics of wild Sola-num pimpinellifolium core collection under Na2CO3 stress were evaluated in this study.The results showed that Na2CO3 solution had a significant inhibitory effect on the germination of tomato seeds,but the toler-ance of different resources to Na2CO3 stress was significantly different.Under the condition of high Na2CO3 concentration of 60 mmol L-1,the relative germination potential,relative germination rate and rela-tive germination index were comprehensively identified.A total of 14 saline-alkali tolerant materials such as P8,P9,P12,P13,P25,P30,P40,P50,P51,P53,P56,P61,P62 and P63 and 22 saline-alkali sensitive materials were identified.The relative germination rate and relative germination potential of saline-alkali tolerant materials could reach more than 85％.Among them,the relative germination rate and relative ger-mination potential of P51 and P61 reached 100％,which had strong saline-alkali tolerance.Through the analysis of genome-wide data and the reported salt-tolerant SIScaBP8 gene variation from currant toma-toes,it is speculated that the salt-tolerant traits of these resources are controlled by different genes,and some resources contain new salt-tolerant sites,and salt-tolerant and alkali-tolerant may have different reg-ulatory mechanisms.The results of this study provide a new basis for genetic improvement and mecha-nism analysis of saline-alkali tolerance using wild resources.
关键词
Na2CO3/醋栗番茄/耐盐碱/萌发期/评价Key words
Na2CO3/Solanum pimpinellifolium/saline-alkali tolerance/germination stage/evaluation引用本文复制引用
基金项目
国家自然科学基金(31991185)
农业农村部园艺作物生物学与种质创制重点实验室项目(2018YFD1000801)
中国农业科学院科技创新工程项目(CAASASTIP-IVFCAAS)()
出版年
2024
NETL
NSTL
万方数据