Identification and characterisation of auxin-binding protein gene ABP1 in Chenopodium quinoa
The growth hormone binding protein ABP1 has been shown to function as a growth hormone re-ceptor.Based on ABP1 from Arabidopsis thaliana,we screened the ABP1 gene and protein sequences of quinoa by BLAST,and analyzed its physicochemical properties,subcellular localization,secondary and ter-tiary structure,motif and domain analysis,phylogenetic tree,and promoter cis-acting elements by bioinfor-matics methods.The results showed that:quinoa genome has two genes encoding ABP1,CqABP1.7g and CqABP1.17g,which are located in Chr07 and Chr17;the isoelectric points of CqABP1s are from 6.03 to 6.35,and the relative values of the content of aliphatic side-chain amino acids in the proteins are from 90.88％to 96.22％,and the subcellular localization is in the cytoplasm and endoplasmic reticulum.The secondary structure is dominated by extended chains,irregular curls and α-helices;the ABP1 of quinoa is more closely homologous to that of sugar beet,and evolutionarily diverged into monocotyledons.CqABP1.17g is expressed at a high level in quinoa flowers,whereas CqABP1.7g is expressed at a high level in seed development;multiple insertion deletion(INDEL)variants have been found in CqABP1.17g,only one INDEI variant was detected in CqABP1.7g,and no code-shifting mutation was detected;the predicted interacting proteins included Auxin efflux carrier,Guanine nucleotide exchange factor SPIKE 1,CRIB do-main-containing protein,etc.The results of this study provide a theoretical foundation and experimental basis for further exploring the function of CqABP1s gene and regulating the growth and development of quinoa.
quinoa(Chenopodium quinoa)ABP1gene expressionphylogenycis-acting elements
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