Cloning and functional analysis of GhSTR2 gene of cotton fatty acid transporter in disease resistance
The fatty acid transporter STR can regulate the outward transport of intracellular fatty acids,playing an important role in plant growth,development,and response to biotic stress.The study reported that the STR gene of Tribulus terrestris alfalfa plays an important role in disease resistance of mycorrhizal fungi.To analyze the role of GhSTR gene in cotton response to Fusarium wilt and Verticillium wilt,and to provide genetic resources for cotton molecular breeding for disease resistance.This experiment aligned the homologous sequence of GhSTR2 gene in the cotton genome database based on the sequence of al-falfa STR2 gene,and gene was cloned and the sequence was analyzed.qRT-PCR method was used to de-tect the expression patterns of GhSTR2 gene in cotton at different time points after treatment with Fusari-um wilt and Verticillium wilt,and virus induced gene silencing(VIGS)technology was used to verify the effect of GhSTR2 gene in cotton resistance to Fusarium wilt and Verticillium wilt.The results showed that the cloned GhSTR2 gene,encods 724 amino acids,has an open reading frame of 2 175 bp,and was pre-dicted to be an unstable transmembrane protein containing an ABC_TRANSPORTER domain.The real-time qRT-PCR analysis showed that there was a significant difference in the relative expression level of GhSTR2 gene after treatment by pathogenic bacteria of Verticillium wilt and Fusarium wilt respectively compared with the control.Compared with the negative control plant TRV:00,cotton plants with the GhSTR2 gene si-lenced by VIGS showed a significant improvement in disease resistance.The disease index,degree of vas-cular bundle browning detected by stem dissection,efficiency of infected stem segments,and number of fungal hyphae in the silenced plants were significantly lower than those in the control group.The above re-sults indicate that the GhSTR2 gene is involved in the defense process of cotton against Fusarium wilt and Verticillium wilt,and is a negative correlation factor in cotton's disease resistance response.
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