Identification of an ABA-insensitive mutant in Arabidopsis thaliana by genome resequencing
Abscisic acid(ABA)is one of the important plant hormones in regulating seed dormancy and germination.In this study,an ABA-insensitive mutant abi10(ABA-insensitive mutant 10)was identified from a T-DNA insertion mutant library of Arabidopsis thaliana.Genetic analysis shows that the phenotype of the abi10 mutant is controlled by a nucleus-encoded gene.Three T-DNA insertion sites were obtained by rese-quencing and analyzing the genome of abi10 mutant.Interestingly,one of the candidate T-DNA insertion sites is located in the promoter region of ABI4,which is an important transcription factor in the ABA signal-ing pathway.The F1 progenies from the hybridization of abi10 and abi4 mutants displayed an ABA-insensi-tive phenotype.Moreover,real-time quantitative PCR analysis revealed that the expression of ABI4 gene in abi10 mutant was significantly reduced compared to that of wild type.In conclusion,abi10 was identified as a new mutant of ABI4 gene by genome resequencing technology,which would provide new research materials for further studies on the regulation of ABI4 gene expression.
ABA insensitivityABI4allelic mutationresequencing technology
NETL
NSTL
万方数据
