利用基因组重测序技术鉴定一个拟南芥ABA不敏感突变体
Identification of an ABA-insensitive mutant in Arabidopsis thaliana by genome resequencing
曾馨篁 1陈舒静 1刘雨洁 1谢欣珏 1梁滢 1黄晓琳 1吴建新1
作者信息
- 1. 华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州 510631
- 折叠
摘要
脱落酸(ABA)是调控种子休眠与萌发过程的重要植物激素之一.本研究通过筛选拟南芥(Arabidop-sis thaliana)T-DNA插入突变体库,获得一株ABA不敏感突变体abi10(ABA-insensitive mutant 10).遗传分析表明,该突变体受核编码的隐性单基因控制.对abi10突变体植株进行基因组重测序,通过序列比对分析发现abi10突变体基因组中有3个T-DNA插入位点,其中有一个插入位点位于编码ABA信号转导途径中重要转录因子ABI4的基因启动子区.通过与abi4突变体做杂交进行等位突变验证,发现杂交F1代表现为ABA不敏感的隐性表型.进一步通过实时荧光定量PCR检测,发现abi10突变体中ABI4基因的表达量显著降低.综上所述,本文利用基因组重测序技术,鉴定到的abi10是一个新的ABI4基因等位突变体,研究结果为后续深入研究ABI4基因的表达调控机制提供研究材料.
Abstract
Abscisic acid(ABA)is one of the important plant hormones in regulating seed dormancy and germination.In this study,an ABA-insensitive mutant abi10(ABA-insensitive mutant 10)was identified from a T-DNA insertion mutant library of Arabidopsis thaliana.Genetic analysis shows that the phenotype of the abi10 mutant is controlled by a nucleus-encoded gene.Three T-DNA insertion sites were obtained by rese-quencing and analyzing the genome of abi10 mutant.Interestingly,one of the candidate T-DNA insertion sites is located in the promoter region of ABI4,which is an important transcription factor in the ABA signal-ing pathway.The F1 progenies from the hybridization of abi10 and abi4 mutants displayed an ABA-insensi-tive phenotype.Moreover,real-time quantitative PCR analysis revealed that the expression of ABI4 gene in abi10 mutant was significantly reduced compared to that of wild type.In conclusion,abi10 was identified as a new mutant of ABI4 gene by genome resequencing technology,which would provide new research materials for further studies on the regulation of ABI4 gene expression.
关键词
ABA不敏感/ABI4/等位突变/重测序技术Key words
ABA insensitivity/ABI4/allelic mutation/resequencing technology引用本文复制引用
出版年
2024
NETL
NSTL
万方数据