Morphological,physiological and biochemical characterization during the formation of adventitious roots in Eleutherococcus giraldii
This study analyzed the changing patterns of morphology,physiology and biochemistry during the development of adventitious roots of Eleutherococcus giraldii to provide theoretical support for im-proving its propagation by cutting technology.Using the annual cuttings of E.giraldii as the test materi-al,the adventitious root morphology,nutrient content,antioxidant enzyme activity,and phytohormone content during the rooting process were measured.The results showed that no latent root primordia were found at the base of E.giraldii,which belonged to the induced rooting type,and the cuttings were rooted from both cortical and healing tissue,which belonged to the mixed rooting type.With the forma-tion of adventitious roots,the content of soluble protein and soluble sugar was lower during the non-cal-lus formation stage(phase Ⅰ),callus formation stage(phase Ⅱ),and adventitious root occurrence stage(phase Ⅲ),and increased to the highest during the root extension stage(phase Ⅳ).The activity of super-oxide dismutase(SOD)continued to increase from phase Ⅰ to phase Ⅳ,while the activities of peroxidase(POD)and catalase(CAT)showed a trend of first increasing and then decreasing.The content of abscis-ic acid(ABA)and gibberellin 3(GA3)in the bark of cuttings decreased in phase Ⅱ and then stabilized,while the content of indole-3-acetic acid(IAA)decreased in phase Ⅲ and then stabilized.The content of zeatin riboside(ZR)continued to decrease.IAA/ABA remained at a high level in the first three periods and decreased in the fourth period.Overall,the results indicate that E.giraldii belongs to the mixed root-ing type.The increase of soluble protein content,soluble sugar content and SOD activity was conducive to the formation of adventitious roots,the increase of POD and CAT activities was conducive to the for-mation of callus,and a small amount of IAA and GA3were conducive to the differentiation of adventi-tious roots at the base of cuttings.
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