Genome-wide identification and expression analysis of AP2/ERF transcription factor family in Baphicacanthus cusia
Indigo and indirubin are the major indole alkaloids in Baphicacanthus cusia,which have the dual value attributes of pharmacodynamic substances and natural pigments,and have high economic and so-cial benefits.The AP2/ERF family genes can regulate the synthesis of secondary metabolites at the tran-scriptional level.In this study,205 AP2/ERF transcription factors were identified from the genome of B.cu-sia using bioinformatics,which were unevenly distributed on 16 chromosomes.The members were subdivided into 28 AP2s,103 ERFs,65 DREBs,7 RAVs and 2 Soloist subfamilies by domain characteriza-tion and phylogenetic analysis.Conserved motif and gene structure analysis showed that Motif 1,2,4,and 5 were widely distributed in B.cusia AP2/ERF,some subfamilies had their own specific Motif,and the in-tron/exon structure of each group was consistent.Interspecies collinearity analysis found that 49.8％of the B.cusia AP2/ERFs had homologous gene pairs in Arabidopsis thaliana.The differentially expressed genes in tissues in response to methyl jasmonate induction were screened in combination with the tran-scriptome,which provided a reference for further research of the role of AP2/ERF in the regulation of syn-thesis of indole alkaloids.
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