Identification of mulberry root exudates and effects of main active compounds on the growth of Ralstonia pseudosolanacearum
To investigate the antibacterial activity of mulberry(Morus alba)root exudates on the Ralstonia pseudosolanacearum,this study utilized gas chromatography-mass spectrometry technology and plate in-hibition assays to examine the effect of the main active substances in mulberry root exudates on the growth of R.pseudosolanacearum.The results indicate that the predominant active substances in mulber-ry root exudates,including(Z)-13-docosenamide and(Z)-9-octadecenamide(42.6%),and(endo)-(-)-3-bro-mocamphor(18.33%),exhibited significant inhibitory effects on tne growth of the mulberry bacterial wilt of MRS-5.The best inhibitory effect was observed at a concentration of 1.5 g·L-1,with inhibition rates exceed-ing 90%.Moreover,these substances were able to significantly inhibit the biofilm formation and motility of MRS-5.This study reveals the inhibitory effects and potential control mechanisms of the main active sub-stances in mulberry root exudates,contributing important scientific value and practical significance to the understanding and management of mulberry wilt disease.