To investigate the antibacterial activity of mulberry(Morus alba)root exudates on the Ralstonia pseudosolanacearum,this study utilized gas chromatography-mass spectrometry technology and plate in-hibition assays to examine the effect of the main active substances in mulberry root exudates on the growth of R.pseudosolanacearum.The results indicate that the predominant active substances in mulber-ry root exudates,including(Z)-13-docosenamide and(Z)-9-octadecenamide(42.6%),and(endo)-(-)-3-bro-mocamphor(18.33%),exhibited significant inhibitory effects on tne growth of the mulberry bacterial wilt of MRS-5.The best inhibitory effect was observed at a concentration of 1.5 g·L-1,with inhibition rates exceed-ing 90%.Moreover,these substances were able to significantly inhibit the biofilm formation and motility of MRS-5.This study reveals the inhibitory effects and potential control mechanisms of the main active sub-stances in mulberry root exudates,contributing important scientific value and practical significance to the understanding and management of mulberry wilt disease.