首页|耐盐碱解磷菌Bacillus sp.DYS211对土壤磷溶解和植物生长的促进作用

耐盐碱解磷菌Bacillus sp.DYS211对土壤磷溶解和植物生长的促进作用

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土壤全磷含量高而速效磷含量不足严重制约盐碱地区磷的高效利用.虽然耐盐碱解磷菌可以溶解不溶性磷,但很少有研究关注其在植物生长中的应用.我们从盐碱地区鸟粪中分离到一株解磷菌,鉴定命名为Bacillus sp.DYS211,并对其生长特性和耐盐碱能力进行了测定.为研究解磷菌对植物种子萌发和生长的影响,在盐地碱蓬中进行解磷菌添加的盆栽试验.该解磷菌菌株在培养前12 h生长迅速,48 h时溶磷量最大,达到258.22 mg L-1.耐盐碱性和解磷能力试验表明,DYS211倾向于溶解无机磷,为嗜盐菌,在1％-8％盐度条件下具有良好的解磷效果(有效磷含量＞150 mg L-1).在葡萄糖和蔗糖(C源)以及硫酸铵、硝酸铵和酵母浸膏粉(N源)中均表现出较好的解磷能力.在促生长试验中,该解磷菌对种子萌发有促进作用,特别是在高盐胁迫下,促进率为8.33％.在盐碱条件下,解磷菌均能显著提高盐地碱蓬的株高和生物量(最高可达3倍),且高盐胁迫下茎粗增加.该菌株表现出在盐碱地区植被恢复中的潜力.
Soil P solubilization and plant growth promotion by a saline-alkali-tolerant P-solubilizing bacterium,Bacillus sp.DYS211
High total P content but insufficient available P in soil is an obstacle that restricts the efficient utilization of P in saline-alkali soil regions.Although saline-alkali resistant P-solubilizing bacteria(PSB)solubilize insoluble P,few studies have focused on their application in plant growth.We isolated a PSB strain,identified as Bacillus sp.DYS211,from bird droppings in saline-alkali regions and determined its growth characteristics and resistance to salt and alkalis.To investigate the effect of PSB on the germination and growth of plant seeds,we performed a potting experiment using Suaeda salsa with PSB added.The PSB strain grew rapidly in the first 12 h,and the solubilized P content from PSB reached a maximum of 258.22 mg L-1 at 48 h.Saline-alkali tolerance and P-solubilizing ability tests showed that Bacillus sp.DYS211 preferred to dissolve inorganic P,was halophilic,and had a good P-solubilizing effect at 1％-8％salinity(available P＞150 mg L-1).It exhibited good P solubilization abilities when glucose and sucrose were used as C sources or when ammonium sulfate,ammonium nitrate or yeast extract powder were used as N sources.In the growth promotion test,PSB increased seed germination,particularly under high-salinity stress,with a growth promotion of 8.33％.The PSB also improved the growth of S.salsa,including plant height and biomass(up to three times)under both saline and alkaline conditions,and the stem diameter increased under high-salinity stress.This strain demonstrates potential for vegetation restoration in saline-alkali regions.

available phosphorusphosphate-solubilizing bacteriaSuaeda salsaseed germinationplant biomass

Wei Wang、Xiaodan Sun、Wenhao Huang、Xiaoting Men、Shijie Yi、Fengrong Zheng、Zhaohui Zhang、Zongling Wang

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Marine Ecology Research Center,First Institute of Oceanography,Ministry of Natural Resources,Qingdao 266061,China

Laboratory of Marine Ecological Environment Science and Engineering,Ministry of Natural Resources,Qingdao 266061,China

Qingdao Pilot National Laboratory for Marine Science and Technology,Qingdao 266061,China

Environmental Science and Engineering,Ocean University of China,Qingdao 266061,China

Institute of Metabolism and Integrative Biology,Fudan University,Shanghai 200438,China

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有效磷 解磷菌 盐地碱蓬(Suaeda salsa) 种子萌发 植物生物量

CNOOC Marine Environmental and Ecological Protection Public Welfare FoundationResearch Project of China Association of Marine AffairsQingdao Postdoctoral Foundation

CF-MEEC/TR/2021-112017AA03QDBSH202109

2023

10.1093/jpe/rtad028

植物生态学报(英文版)

植物生态学报(英文版)

CSCD
ISSN：1752-9921
年,卷(期)：2023.(6)
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