Leaf carbon, nitrogen and phosphorus stoichiometry at different growth stages in dominant tree species of a monsoon broad-leaved evergreen forest in Pu'er, Yunnan Province, China
Aims Our objective was to explore the effect of growth stage on leaf C∶N∶P stoichiometry in dominant species of a monsoon broad-leaved evergreen forest in Pu'er,Yunnan Province.Methods We collected leaves from six dominant species (i.e.Castanopsis hystrix,C.echidnocarpa,Lithocarpus fenestratus,L.truncatus,Schima wallichii and Anneslea fragrans) at different growth stages from seedlings to mature trees.The leaf N,P and C contents were measured.The effects of species,growth stage and their interaction on leaf C,N and P contents and stoichiometry were analyzed by Repeated-Measure ANOVA in SPSS 19.0.Important findings The ranges of leaf N,P and C contents and mass ratios of C∶N,N∶P and C∶P in the six species across different growth stages were 7.90-17.72 mg·g-1,0.34-1.39 mg·g-1,458.48-516.87 mg·g-1,28.04-65.70,11.41-63.50 and 355.23-1 878.17,respectively.The coefficient of variation (CV) for N∶P mass ratio ranged between 19.19%-91.65%,with an average of 36.46%; CV for C∶P varied between 15.99%-91.60%,with an average of 34.80%; whilst CVfor C varied between 1.61%-5.89%,with an average of 3.12%.Factorial analysis of the variation for each variable,with growth stage and species as independent factors,showed that leaf N and C contents and the mass ratios of leaf C∶N,N∶P and C∶P were mainly determined by growth stage,while leaf P content was mainly determined by an interaction between growth stage and species.
stoichiometrydominant speciesmonsoon broad-leaved evergreen forestinteractioncoefficient of variation