Effects of Penicillium oxalicum C11 on Rehmannia glutinosa growth and its metabolites analysis
Aims The aim of this study was to isolate the harmful strain and analyze the effects of its metabolites on Rehmannia glutinosa,which provided theoretical support for solving R.glutinosa continuous cropping obstacles.Methods The fungi were isolated from continuous cropping soil.The fungal fermentation liquid metabolites were separated using different polarity gradient extraction and silica gel column chromatography.Metabolites were determined by high performance liquid chromatography and liquid chromatography-mass spectrometry.Important findings A strain of Penicillium oxalicum C11 was isolated from continuous cropping soil.The toxic metabolite of P.oxalicum C11 was identified as cyclo(L-Arg-L-Leu)dipeptide(molecular weight:269.347,molecular formula:C12H23N5O2).Results indicated P.oxalicum C11 inhibited R.glutinosa yield by 41.5％,suggesting that the toxic metabolite L-Arg-L-Leu could be vital for R.glutinosa continuous cropping obstacles.
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