长春花激素完全适应型细胞的生长和阿玛碱合成特性
Cell Growth and Ajmalicine Accumulation in a Full Habituated Catharanthus roseus Cell Line C20hi
郑珍贵 1周煜 1刘涤 1胡之璧1
作者信息
- 1. 上海中医药大学中药研究所,上海,200032
- 折叠
摘要
从长春花激素依赖型细胞系(C20D)筛选出一种激素完全适应型的细胞系(C20hi),考察了两种细胞生长、阿玛碱合成和与吲哚生物碱生物合成相关的酶的活性,结果表明:在生长培养基上二者生长无显著差异,而C20hi细胞平均阿玛碱含量是C20D的31.9倍,在生产培养基上C20hi细胞生长较C20D快,C20hi平均阿玛碱含量是C20D的18.4倍.通过比较生产和生长培养基中C20hi细胞的色氨酸脱羧酶、异胡豆苷合酶和牛儿醇-10-脱氢酶活性,发现在生产培养基中培养细胞的3种酶活性均显著高于生长培养基,但与阿玛碱积累没有密切关系.研究结果还表明,通过5年的继代培养,激素完全适应型细胞系C20hi的阿玛碱含量是比较稳定的.
Abstract
A full habituated cell line C20hi was screened from 2,4-D dependent line (C20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C20hi cells grew faster than C20D cells, and average ajmalicine content in C20hi cells was 18.4 times more than that in C20D when cultured in the production medium. In the growth medium, average ajmalicine content in C20hi cells was 31.9 times more than that in C20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C20hi and C20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol-10-dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C20hi cells are relatively stable in five years of culture.
关键词
完全适应型细胞/阿玛碱/细胞培养/长春花Key words
full habituated cells/ajmalicine/cell culture/Catharanthus roseus引用本文复制引用
基金项目
国家中医药管理局青年基金(97Y010)
上海市教委资助项目(94009)
出版年
2002
NETL
NSTL
维普
万方数据