首页|Seed metabolomic study reveals significant metabolite variations and correlations among different soybean cultivars
Seed metabolomic study reveals significant metabolite variations and correlations among different soybean cultivars
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Soybean [Glycine max (L.) Merr.] is one of the world’s major crops, and soybean seeds are a rich and important resource for proteins and oils. While “omics”studies, such as genomics, transcriptomics, and proteomics, have been widely applied in soybean molecular research, fewer metabolomic studies have been conducted for large-scale detection of low molecular weight metabolites, especial y in soybean seeds. In this study, we investigated the seed metabolomes of 29 common soybean cultivars through combined gas chromatography-mass spectrometry and ultra-performance liquid chromatography-tandem mass spectrometry. One hundred sixty-nine named metabolites were identified and subsequently used to construct a metabolic network of mature soybean seed. Among the 169 detected metabolites, 104 were found to be significantly variable in their levels across tested cultivars. Metabolite markers that could be used to distinguish genetical y related soybean cultivars were also identified, and metabolite-metabolite correlation analysis revealed some significant associations within the same or among different metabolite groups. Findings from this work may potentially provide the basis for further studies on both soybean seed metabolism and metabolic engineering to improve soybean seed quality and yield.
Gas chromatography-mass spectrometry (GC-MS)metabolic networkmetabolite-metabolite correlationmetabolomicssoybeanultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
Hong Lin、Jun Rao、Jianxin Shi、Chaoyang Hu、Fang Cheng、Zoe AWilson、Dabing Zhang、Sheng Quan
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National Center for the Molecular Characterization of Geneticaly Modified 0rganisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Department of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
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