首页|Getting started in mapping-by-sequencing

Getting started in mapping-by-sequencing

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Next-generation sequencing (NGS) technologies al ow the cost-effective sequencing of whole genomes and have expanded the scope of genomics to novel applications, such as the genome-wide characterization of intraspecific polymorphisms and the rapid mapping and identification of point mutations. Next-generation sequencing platforms, such as the Il umina HiSeq2000 platform, are now commercial y available at affordable prices and routinely produce an enormous amount of sequence data, but their wide use is often hindered by a lack of knowledge on how to manipulate and process the information produced. In this review, we focus on the strategies that are available to geneticists who wish to incorporate these novel approaches into their research but who are not familiar with the necessary bioinformatic concepts and computational tools. In particular, we comprehensively summarize case studies where the use of NGS technologies has led to the identification of point mutations, a strategy that has been dubbed“mapping-by-sequencing”, and review examples from plants and other model species such as Caenorhabditis elegans, Saccharomyces cerevisiae, and Drosophila mela-nogaster. As these technologies are becoming cheaper and more powerful, their use is also expanding to al ow mutation identification in species with larger genomes, such as many crop plants.

Mapping-by-sequencingmassively paral el sequencingmutation identificationSHOREmappingwhole-genome re-sequencing

Héctor Candela、Rubén Casanova-Sáez、José Luis Micol

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Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202, Elche, Spain

Department of Forest Genetics and Plant Physiology, Ume? Plant Science Center, Swedish University of Agricultural Sciences, Umea SE-901 83, Sweden

Ministerio de Economía y Competitividad of SpainMinisterio de Economía y Competitividad of SpainGeneralitat ValencianaMarie Curie International Reintegration GrantMinisterio de Economía y Competitividad of SpainMinisterio de Economía y Competitividad of Spain

BFU2011-22825CSD2007-00057TRANSPLANTAPROMETEOII/2014/003PIRG03-GA-2008-231073BFU2012-31719BES-2009-014106

2015

10.1111/jipb.12305

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCDCSCDSCI
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2015.(7)
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