首页|Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis

Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Hydrogen sulfide (H2S) is an important gaseous molecule in various plant developmental processes and plant stress responses. In this study, the transgenic Arabidopsis thaliana plants with modulated expressions of two cysteine desulfhydrases, and exogenous H2S donor (sodium hydrosulfide, NaHS) and H2S scavenger (hypotaurine, HT) pre-treated plants were used to dissect the involvement of H2S in plant stress responses. The cysteine desulfhydrases overexpressing plants and NaHS pre-treated plants exhibited higher endogenous H2S level and improved abiotic stress tolerance and biotic stress resistance, while cysteine desulfhydrases knockdown plants and HT pre-treated plants displayed lower endogenous H2S level and decreased stress resistance. Moreover, H2S upregulated the transcripts of multiple abiotic and biotic stress-related genes, and inhibited reactive oxygen species (ROS) accumulation. Interest-ingly, MIR393-mediated auxin signaling including MIR393a/b and their target genes (TIR1, AFB1, AFB2, and AFB3) was transcrip-tional y regulated by H2S, and was related with H2S-induced antibacterial resistance. Moreover, H2S regulated 50 carbon metabolites including amino acids, organic acids, sugars, sugar alcohols, and aromatic amines. Taken together, these results indicated that cysteine desulfhydrase and H2S conferred abiotic stress tolerance and biotic stress resistance, via affecting the stress-related gene expressions, ROS metabolism, metabolic homeostasis, and MIR393-targeted auxin receptors.

Abiotic stressauxin receptorbiotic stresscysteine desulfhydrasehydrogen sulfideMIR393

Haitao Shi、Tiantian Ye、Ning Han、Hongwu Bian、Xiaodong Liu、Zhulong Chan

展开 >

Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan 430074, China

Institute of Genetics, State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China

College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China

National Natural Science Foundation of China“the Hundred Talents Program”“the Hundred Talents Program”National Natural Science Foundation of ChinaYouth Innovation Promotion Association of Chinese Academy of SciencesKnowledge Innovative Key Program of Chinese Academy of Sciences

3137030254Y154761O0107629Y329631O02633120019429Y429371O043755Y455446O0544

2015

10.1111/jipb.12302

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCDCSCDSCI
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2015.(7)
  • 22
  • 59