Post-transcriptional regulation of grain weight and shape by the RBP-A-J-K complex in rice

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外文摘要：RNA-binding proteins(RBPs)are components of the post-transcriptional regulatory system,but their reg-ulatory effects on complex traits remain unknown.Using an integrated strategy involving map-based cloning,functional characterizations,and tran-scriptomic and population genomic analyses,we re-vealed that RBP-K(LOC_Os08g23120),RBP-A(LO-C_Os11g41890),and RBP-J(LOC_Os10g33230)encode proteins that form an RBP-A-J-K complex that negatively regulates rice yield-related traits.Ex-aminations of the RBP-A-J-K complex indicated RBP-K functions as a relatively non-specific RBP chaperone that enables RBP-A and RBP-J to func-tion normally.Additionally,RBP-J most likely affects GA pathways,resulting in considerable increases in grain and panicle lengths,but decreases in grain width and thickness.In contrast,RBP-A negatively regulates the expression of genes most likely in-volved in auxin-regulated pathways controlling cell wall elongation and carbohydrate transport,with substantial effects on the rice grain filling process as well as grain length and weight.Evolutionarily,RBP-K is relatively ancient and highly conserved,whereas RBP-J and RBP-A are more diverse.Thus,the RBP-A-J-K complex may represent a typical functional model for many RBPs and protein complexes that function at transcriptional and post-transcriptional levels in plants and animals for increased functional consistency,efficiency,and versatility,as well as in-creased evolutionary potential.Our results clearly demonstrate the importance of RBP-mediated post-transcriptional regulation for the diversity of complex traits.Furthermore,rice grain yield and quality may be enhanced by introducing various complete or partial loss-of-function mutations to specific RBP genes using clustered regularly interspaced palin-dromic repeats(CRISPR)/CRISPR-associated protein 9 technology and by exploiting desirable natural tri-genic allelic combinations at the loci encoding the components of the RBP-A-J-K complex through marker-assisted selection.

外文关键词：

GL11grain weight and shapepost-transcriptional regulationRNA-binding proteins

作者：

Ding Ren、Hui Liu、Xuejun Sun、Fan Zhang、Ling Jiang、Ying Wang、Ning Jiang、Peiwen Yan、Jinhao Cui、Jinshui Yang、Zhikang Li、Pingli Lu、Xiaojin Luo

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作者单位：

State Key Laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology,School of Life Sciences,Fudan University,Shanghai 200438,China

State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,China

MOE Key Laboratory of Crop Physiology,Ecology and Genetic Breeding College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China

State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,China

College of Agronomy,Anhui Agricultural University,Hefei 230036,China

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基金：

Innovation Program of Shanghai Municipal Education CommissionNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShanghai Science and Technology Innovation Action Plan ProjectInnovation Program of Chinese Academy of Agricultural SciencesNational Key Research and Development Program of China

项目编号：

2023ZKZD0532172043319719183217035622N119002002021YFA1300401

出版年：

2024

DOI：

10.1111/jipb.13583

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(1)

参考文献量68