首页|A centromere map based on super pan-genome highlights the structure and function of rice centromeres

A centromere map based on super pan-genome highlights the structure and function of rice centromeres

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary factors.Rice centromeres are crucial for chromo-some segregation,and contain some unreported genes.Due to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is needed.We constructed a high-quality centromere map based on the rice super pan-genome consisting of a 251-accession panel com-prising both cultivated and wild species of Asian and African rice.We showed that rice cen-tromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary patterns.We also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and evolution.Fur-thermore,high-quality genome assembly and com-plete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome in-formation despite mapping and cloning of cen-tromere genes being challenging.We investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regu-lates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9 methods.By revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement.

centromeresuper pan-genomeCentO satellite repeatrice

Yang Lv、Congcong Liu、Xiaoxia Li、Yueying Wang、Huiying He、Wenchuang He、Wu Chen、Longbo Yang、Xiaofan Dai、Xinglan Cao、Xiaoman Yu、Jiajia Liu、Bin Zhang、Hua Wei、Hong Zhang、Hongge Qian、Chuanlin Shi、Yue Leng、Xiangpei Liu、Mingliang Guo、Xianmeng Wang、Zhipeng Zhang、Tianyi Wang、Bintao Zhang、Qiang Xu、Yan Cui、Qianqian Zhang、Qiaoling Yuan、Noushin Jahan、Jie Ma、Xiaoming Zheng、Yongfeng Zhou、Qian Qian、Longbiao Guo、Lianguang Shang

展开 >

State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China

Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China

Yazhouwan National Laboratory,No.8 Huanjin Road,Yazhou District,Sanya City 572024,China

National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China

展开 >

centers of Agricultural Genomics Institute at Shenzhenhighperformance computing centers of China National Rice Research InstituteNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaInnovation Program of Chinese Academy of Agricultural SciencesYouth Innovation of Chinese Academy of Agricultural SciencesGuangdong Basic and Applied Basic Research FoundationYouth Program of Guangdong Basic and Applied Research

3218810232372148Y20230C362023B15150200532021A1515111123

2024

10.1111/jipb.13607

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(2)
  • 51