首页|The BTB/TAZ domain-containing protein CmBT1-mediated CmANR1 ubiquitination negatively regulates root development in chrysanthemum
The BTB/TAZ domain-containing protein CmBT1-mediated CmANR1 ubiquitination negatively regulates root development in chrysanthemum
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Roots are fundamental for plants to adapt to variable environmental conditions.The develop-ment of a robust root system is orchestrated by numerous genetic determinants and,among them,the MADS-box gene ANR1 has garnered substantial attention.Prior research has demon-strated that,in chrysanthemum,CmANR1 positively regulates root system development.Nevertheless,the upstream regulators involved in the CmANR1-mediated regulation of root devel-opment remain unidentified.In this study,we successfully identified bric-a-brac,tramtrack and broad(BTB)and transcription adapter putative zinc finger(TAZ)domain protein CmBT1 as the interacting partner of CmANR1 through a yeast-two-hybrid(Y2H)screening library.Furthermore,we validated this physical interaction through bimolecular fluorescence complementation and pull-down assays.Functional assays revealed that CmBT1 exerted a negative influence on root development in chrysanthemum.In both in vitro and in vivo assays,it was evident that CmBT1 mediated the ubiquitination of CmANR1 through the ubiquitin/26S proteasome pathway.This ubiquitination subsequently led to the degrada-tion of the CmANR1 protein and a reduction in the transcription of CmANR1-targeted gene CmPIN2,which was crucial for root development in chrysanthemum.Genetic analysis suggested that CmBT1 modulated root development,at least in part,by regulating the level of CmANR1 protein.Collectively,these findings shed new light on the regulatory role of CmBT1 in degrading CmANR1 through ubiquitination,thereby repressing the expression of its targeted gene and inhibiting root development in chrysanthemum.
chrysanthemumMADS-box transcription factorroot development
Lian-Da Du、Zhang-Ji Guan、Yan-Hong Liu、Hui-Dong Zhu、Quan Sun、Da-Gang Hu、Cui-Hui Sun
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Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China
National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShandong Province
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