首页|Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome

Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome

扫码查看
原文链接
  • 万方数据
ChinaMu is the largest sequence-indexed Mutator(Mu)transposon insertional library in maize(Zea mays).In this study,we made significant im-provements to the size and quality of the ChinaMu library.We developed a new Mu-tag isolation method Mu-Tn5-seq(MuT-seq).Compared to the previous method used by ChinaMu,MuT-seq re-covered 1/3 more germinal insertions,while re-quiring only about 1/14 of the sequencing volume and 1/5 of the experimental time.Using MuT-seq,we identified 113,879 germinal insertions from 3,168 Mu-active F1 families.We also assembled a high-quality genome for the Mu-active line Mu-starter,which harbors the initial active MuDR el-ement and was used as the pollen donor for the mutation population.Using the Mu-starter ge-nome,we recovered 33,662(15.6％)additional germinal insertions in 3,244(7.4％)genes in the Mu-starter line.The Mu-starter genome also im-proved the assignment of 117,689(54.5％)ger-minal insertions.The newly upgraded ChinaMu dataset currently contains 215,889 high-quality germinal insertions.These insertions cover 32,224 pan-genes in the Mu-starter and B73Ref5 ge-nomes,including 23,006(80.4％)core genes shared by the two genomes.As a test model,we investigated Mu insertions in the pentatricopep-tide repeat(PPR)superfamily,discovering in-sertions for 92％(449/487)of PPR genes in Chi-naMu,demonstrating the usefulness of ChinaMu as a functional genomics resource for maize.

ChinaMuMutatorMuT-seqPPR geneZea mays

Lei Liang、Yuancong Wang、Yanbin Han、Yicong Chen、Mengfei Li、Yibo Wu、Zeyang Ma、Han Zhao、Rentao Song

展开 >

State Key Laboratory of Maize Bio-breeding,Frontiers Science Center for Molecular Design Breeding,Joint International Research Laboratory of Crop Molecular Breeding,National Maize Improvement Center,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100091,China

Institute of Crop Germplasm and Biotechnology,Jiangsu Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

Sanya Institute of China Agricultural University,Sanya 572025,China

Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China

展开 >

2024

10.1111/jipb.13637

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(4)
  • 47