首页|OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

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Due to its tropical origins,rice(Oryza sativa)is susceptible to cold stress,which poses severe threats to production.OsNAC5,a NAC-type tran-scription factor,participates in the cold stress re-sponse of rice,but the detailed mechanisms remain poorly understood.Here,we demonstrate that OsNAC5 positively regulates cold tolerance at ger-mination and in seedlings by directly activating the expression of ABSCISIC ACID INSENSITIVE 5(OsABI5).Haplotype analysis indicated that single nucleotide polymorphisms in a NAC-binding site in the OsABI5 promoter are strongly associated with cold tolerance.OsNAC5 also enhanced OsABI5 stability,thus regulating the expression of cold-responsive(COR)genes,enabling fine-tuned control of OsABI5 action for rapid,precise plant responses to cold stress.DNA affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression,including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A(OsDREB1A),OsMYB20,and PEROX-IDASE 70(OsPRX70).In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription,with marked COR upregulation in OsNAC5-overexpressing lines and down-regulation in osnac5 and/or osabi5 knockout mu-tants.This study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5-CORs transcription module,which may be used to ameliorate cold tolerance in rice via ad-vanced breeding.

ABA signalingABI5cold toleranceDREBNAC5rice

Ruiqing Li、Yue Song、Xueqiang Wagn、Chenngfan Zhgeng、Bo Liu、Huali Zhang、Jian Ke、Xuejing Wu、Liquan Wu、Ruifang Yang、Meng Jiang

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College of Agronomy,Anhui Agricultural University,Hefei 230036,China

Hainan Institute,Yazhou Bay Sci-Tech City,Zhejiang University,Sanya 572025,China

National Key Laboratory of Rice Biology,Advanced Seed Institute,Zhejiang University,Hangzhou 311225,China

State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 311401,China

Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China

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国家自然科学基金国家自然科学基金Research Startup Funding from Hainan Institute of Zhejiang Universitythe"Nanhai New Star"Technology Innovation Talent Platform Project of Hainan ProvincePhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City中央高校基本科研业务费专项Agriculture Research System of Shanghai,China

32071946322018950201-6602-A12203NHXXRCXM202362HSPHDSRF-2023-04-018226-2022-00012202203

2024

10.1111/jipb.13585

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(4)
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