An alfalfa MYB-like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3-mediated degradation
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Drought is a major threat to alfalfa(Medicago sativa L.)production.The discovery of important alfalfa genes regulating drought response will facilitate breeding for drought-resistant alfalfa cultivars.Here,we report a genome-wide association study of drought resistance in alfalfa.We identified and functionally characterized an MYB-like tran-scription factor gene(MsMYBH),which increases the drought resistance in alfalfa.Compared with the wild-types,the biomass and forage quality were enhanced in MsMYBH overexpressed plants.Combined RNA-seq,proteomics and chromatin immunoprecipitation analysis showed that MsMYBH can directly bind to the promoters of MsMCP1,MsMCP2,MsPRX1A and MsCARCAB to improve their expression.The outcomes of such interactions include better water balance,high photosynthetic efficiency and scavenge excess H2O2 in response to drought.Furthermore,an E3 ubiquitin ligase(MsWAV3)was found to induce MsMYBH degrada-tion under long-term drought,via the 26S protea-some pathway.Furthermore,variable-number tandem repeats in MsMYBH promoter were char-acterized among a collection of germplasms,and the variation is associated with promoter activity.Collectively,our findings shed light on the functions of MsMYBH and provide a pivotal gene that could be leveraged for breeding drought-resistant alfalfa.This discovery also offers new insights into the mechanisms of drought resistance in alfalfa.
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