Abstract
The high-affinity potassium transporters(HKTs),selectively permeable to either Na+alone or Na+/K+,play pivotal roles in maintaining plant Na+/K+homeostasis.Although their involvement in salt tolerance is widely reported,the molec-ular underpinnings of Oryza sativa HKTs remain elusive.In this study,we elucidate the structures of OsHKT1;1 and OsHKT2;1,representing two distinct classes of rice HKTs.The dimeric as-sembled OsHKTs can be structurally divided into four domains.At the dimer interface,a half-helix or a loop in the third domain is coordinated by the C-terminal region of the opposite subunit.Additionally,we present the structures of OsHKT1;5 salt-tolerant and salt-sensitive var-iants,a key quantitative trait locus associated with salt tolerance.The salt-tolerant variant of OsHKT1;5 exhibits enhanced Na+transport capability and displays a more flexible con-formation.These findings shed light on the mo-lecular basis of rice HKTs and provide insights into their role in salt tolerance.
基金项目
国家重点研发计划(2022YFA1303400)
国家自然科学基金(32171188)
Chinese Universities Scientific Fund funds(2023RC012)
Chinese Universities Scientific Fund funds(2023TC190)
Young Elite Scientists Sponsorship Program by China Association for Science and Technology()
China Agriculture University Young Talent Program in Life Science(008)
Pinduoduo-China Agricultural University Research Fund(PC2023B01010)
维普
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