The OsWRKY72-OsAAT30/OsGSTU26 module mediates reactive oxygen species scavenging to drive heterosis for salt tolerance in hybrid rice

扫码查看

原文链接

万方数据
维普

外文摘要：Hybrid rice(Oryza sativa)generally outperforms its inbred parents in yield and stress tolerance,a phenomenon termed heterosis,but the underlying mechanism is not completely understood.Here,we combined transcriptome,proteome,physio-logical,and heterosis analyses to examine the salt response of super hybrid rice Chaoyou1000(CY1000).In addition to surpassing the mean values for its two parents(mid-parent heterosis),CY1000 exhibited a higher reactive oxygen spe-cies scavenging ability than both its parents(over-parent heterosis or heterobeltiosis).Nonadditive expression and allele-specific gene expression assays showed that the glutathione S-transferase gene OsGSTU26 and the amino acid transporter gene OsAAT30 may have major roles in heterosis for salt tolerance,acting in an overdominant fashion in CY1000.Furthermore,we identified OsWRKY72 as a common transcription factor that binds and regulates OsGSTU26 and OsAAT30.The salt-sensitive phenotypes were associated with the OsWRKY72paternal genotype or the OsAAT30maternal genotype in core rice germplasm varieties.OsWRKY72paternal specifically repressed the expression of OsGSTU26 under salt stress,leading to salinity sensitivity,while OsWRKY72-maternal specifically repressed OsAAT30,resulting in salinity tolerance.These results suggest that the OsWRKY72-OsAAT30/OsGSTU26 module may play an important role in heterosis for salt tolerance in an overdominant fashion in CY1000 hybrid rice,providing valuable clues to elucidate the mechanism of heterosis for salinity tolerance in hybrid rice.

外文关键词：

allele-specific gene expression(ASE)heterosishybrid ricenonadditive effect(NAE)salt tolerancetran-scriptome and proteome

作者：

Citao Liu、Bigang Mao、Yanxia Zhang、Lei tian、Biao Ma、Zhuo Chen、Zhongwei Wei、Aifu Li、Ye Shao、Gongye Cheng、Lingling Li、Wenyu Li、Di Zhang、Xiaoping Ding、Jiangxiang Peng、Yulin Peng、Jiwai He、Nenghui Ye、Dingyang Yuan、Chengcai Chu、Meijuan Duan

展开 >

作者单位：

Hunan Provincial Key Laboratory of Stress Biology,College of Agriculture,Hunan Agricultural University,Changsha 410128,China

State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125,China

College of Agriculture,South China Agricultural University,Guangzhou 510642,China

State Key Laboratory of Wheat and Maize Crop Science,Center for Crop Genome Engineering,College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China

College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Department of Computational Medicine and Bioinformatics,University of Michigan,Ann Arbor,Ml 48109 USA

Biobin Data Science Co.,Ltd.,Changsha 410221,China

展开 >

基金：

国家自然科学基金国家自然科学基金National Center of Technology Innovation for Saline-Alkali Tolerant Rice湖南省自然科学基金Science and Technology Innovation Program of Hunan ProvinceChangsha Science and Technology Project

项目编号：

32272050U21A202082022PT10052022JJ300212023NK1010kq2202221

出版年：

2024

DOI：

10.1111/jipb.13640

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(4)

参考文献量68