首页|Potassium transporter OsHAK9 regulates seed germination under salt stress by preventing gibberellin degradation through mediating OsGA2ox7 in rice
Potassium transporter OsHAK9 regulates seed germination under salt stress by preventing gibberellin degradation through mediating OsGA2ox7 in rice
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Soil salinity has a major impact on rice seed germination,severely limiting rice production.Herein,a rice germination defective mutant under salt stress(gdss)was identified by using chemical mutagenesis.The GDSS gene was detected via MutMap and shown to encode potassium transporter OsHAK9.Phenotypic analysis of complementation and mutant lines demonstrated that OsHAK9 was an essential regulator responsible for seed germination under salt stress.OsHAK9 is highly expressed in germinating seed embryos.Ion contents and non-invasive micro-test technology results showed that OsHAK9 restricted K+efflux in salt-exposed germinating seeds for the balance of K+/Na+.Disruption of OsHAK9 significantly re-duced gibberellin 4(GA4)levels,and the ger-mination defective phenotype of oshak9a was partly rescued by exogenous GA3 treatment under salt stress.RNA sequencing(RNA-seq)and real-time quantitative polymerase chain reaction analysis demonstrated that the dis-ruption of OsHAK9 improved the GA-deactivated gene OsGA2ox7 expression in germinating seeds under salt stress,and the expression of OsGA2ox7 was significantly in-hibited by salt stress.Null mutants of Os-GA2ox7 created using clustered,regularly in-terspaced,short palindromic repeat(CRISPR)/CRISPR-associated nuclease 9 approach dis-played a dramatically increased seed germina-tion ability under salt stress.Overall,our results highlight that OsHAK9 regulates seed germi-nation performance under salt stress involving preventing GA degradation by mediating Os-GA2ox7,which provides a novel clue about the relationship between GA and OsHAKs in rice.
National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization,Jiangsu Collaborative Innovation Center for Modem Crop Production,Hainan Yazhou Bay Seed Lab,Jiangsu Province Engineering Research Center of Seed Industry Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
International Research Center for Environmental Membrane Biology,Foshan University,Foshan 528000,China
School of Biological Sciences,University of Western Australia,Crawley WA 6009,Australia
国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金广东省自然科学基金Hainan Yazhou Bay Seed Laboratory
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