首页|SIBEL11 regulates flavonoid biosynthesis,thus fine-tuning auxin efflux to prevent premature fruit drop in tomato

SIBEL11 regulates flavonoid biosynthesis,thus fine-tuning auxin efflux to prevent premature fruit drop in tomato

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Auxin regulates flower and fruit abscission,but how developmental signals mediate auxin transport in abscission remains unclear.Here,we reveal the role of the transcription factor BEL1-LIKE HOMEO-DOMAIN11(SIBEL11)in regulating auxin transport during abscission in tomato(Solanum lyco-persicum).SIBEL11 is highly expressed in the fruit abscission zone,and its expression increases during fruit development.Knockdown of SIBEL11 expression by RNA interference(RNAi)caused premature fruit drop at the breaker(Br)and 3 d post-breaker(Br+3)stages of fruit development.Tran-scriptome and metabolome analysis of SIBEL11-RNAi lines revealed impaired flavonoid biosynthesis and decreased levels of most flavonoids,especially quercetin,which functions as an auxin transport inhibitor.This suggested that SIBEL11 prevents premature fruit abscission by modulating auxin ef-flux from fruits,which is crucial for the formation of an auxin response gradient.Indeed,quercetin treatment suppressed premature fruit drop in SIBEL11-RNAi plants.DNA affinity purification se-quencing(DAP-seq)analysis indicated that SIBEL11 induced expression of the transcription factor gene SIMYB111 by directly binding to its promoter.Chromatin immunoprecipitation-quantitative poly-merase chain reaction and electrophoretic mobility shift assay showed that S.lycopersicum MYELO-BLASTOSIS VIRAL ONCOGENE HOMOLOG111(SIMYB111)induces the expression of the core fla-vonoid biosynthesis genes SICHS1,SICHI,SIF3H,and SIFLS by directly binding to their promoters.Our findings suggest that the SIBEL11-SIMYB111 module modulates flavonoid biosynthesis to fine-tune auxin efflux from fruits and thus maintain an auxin response gradient in the pedicel,thereby preventing premature fruit drop.

abscissionauxinflavonoidSIBEL11tomato

Xiufen Dong、Xianfeng Liu、Lina Cheng、Ruizhen Li、Siqi Ge、Sai Wang、Yue Cai、Yang Liu、Sida Meng、Cai-Zhong Jiang、Chun-Lin Shi、Tianlai Li、Daqi Fu、Mingfang Qi、Tao Xu

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College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China

Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province,College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,China

Key Laboratory for Quality and Safety Control of Subtropical Fruits and Vegetables,Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province,Ministry of Agriculture

Key Laboratory of Protected Horticulture of Ministry of Education,Shenyang 110866,China

Crops Pathology and Genetic Research Unit,United States Department of Agriculture Agricultural Research Service,Washington,DC 20250,USA

Department of Plant Sciences,University of California,Davis,CA 95616,USA

ANGENOVO,Oslo 0753,Norway

Laboratory of Fruit Biology,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China

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国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金

3199118432372687323300973220257732302645

2024

10.1111/jipb.13627

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(4)
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