首页|A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato

A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato

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The structural and functional diversity of plant metabolites is largely created via chemical mod-ification of a basic backbone.However,metabolite modifications in plants have still not been thor-oughly investigated by metabolomics approaches.In this study,a widely targeted metabolite modifi-comics(WTMM)strategy was developed based on ultra-high performance liquid chromatography-quadrupole-linear ion trap(UHPLC-Q-Trap)and UHPLC-Q-Exactive-Orbitrap(UHPLC-QE-Orbitrap),which greatly improved the detection sensitivity and the efficiency of identification of modified metabolites.A metabolite modificomics study was carried out using tomato as a model,and over 34,000 signals with MS2 information were obtained from approximately 232 neutral loss transitions.Unbiased metabolite profiling was also performed by utilizing high-resolution mass spectrometry data to annotate a total of 2,118 metabolites with 125 modification types;of these,165 modified metabolites were identi-fied in this study.Next,the WTMM database was used to assess diseased tomato tissues and 29 biomarkers were analyzed.In summary,the WTMM strategy is not only capable of large-scale detection and quantitative analysis of plant-modified metabolites in plants,but also can be used for plant biomarker development.

LC-MSmetabolic diversitymetabolite modificomicsmodified groupstomato

Jun Yang、Ridong Chen、Chao Wang、Chun Li、Weizhen Ye、Zhonghui Zhang、Shouchuang Wang

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School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication),Hainan University,Sanya 572025,China

School of Tropical Agriculture and Forestry,Hainan University,Haikou 572208,China

国家重点研发计划国家自然科学基金国家自然科学基金Hainan Province Science and Technology Special FundYoung Elite Scientists Sponsorship Program by China Association for Science and TechnologyHainan Provincial Academician Innovation Platform ProjectHainan University Startup Fund

2021YFA09096003210021232101662ZDYF2022XDNY1442019QNRC001HD-YSZX-202004KYQDZR21025

2024

10.1111/jipb.13629

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(4)
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