首页|PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

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Transcriptional regulation plays a key role in the control of seed dormancy,and many transcription factors(TFs)have been documented.However,the mechanisms underlying the interactions between different TFs within a transcriptional complex regu-lating seed dormancy remain largely unknown.Here,we showed that TF PHYTOCHROME-INTERACTING FACTOR4(PIF4)physically interacted with the ab-scisic acid(ABA)signaling responsive TF ABSCISIC ACID INSENSITIVE4(ABI4)to act as a transcriptional complex to promote ABA biosynthesis and signaling,finally deepening primary seed dormancy.Both pif4 and abi4 single mutants exhibited a decreased pri-mary seed dormancy phenotype,with a synergistic effect in the pif4/abi4 double mutant.PIF4 binds to ABI4 to form a heterodimer,and ABI4 stabilizes PIF4 at the protein level,whereas PIF4 does not affect the protein stabilization of ABI4.Subsequently,both TFs independently and synergistically promoted the ex-pression of ABI4 and NCED6,a key gene for ABA anabolism.The genetic evidence is also consistent with the phenotypic,physiological and biochemical analysis results.Altogether,this study revealed a transcriptional regulatory cascade in which the PIF4-ABI4 transcriptional activator complex synerg-istically enhanced seed dormancy by facilitating ABA biosynthesis and signaling.

ABAABI4PIF4seed dormancy and germinationtranscriptional complex

Xiaofeng Luo、Yujia Dai、Baoshan Xian、Jiahui Xu、Ranran Zhang、Muhammad Saad Rehmani、Chuan Zheng、Xiaoting Zhao、Kaitao Mao、Xiaotong Ren、Shaowei Wei、Lei Wang、Juan He、Weiming Tan、Junbo Du、Weiguo Liu、Shu Yuan、Kai Shu

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Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection,School of Ecology and Environment,Northwestern Polytechnical University,Xi'an 710129,China

Research & Development,Institute of Northwestern Polytechnical University in Shenzhen,Shenzhen 518057,China

College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China

Institute of Ecological Agriculture,Sichuan Agricultural University,Chengdu 611130,China

College of Resources,Sichuan Agricultural University,Chengdu 611130,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNatural Science Basic Research Program of ShaanxiShaanxi Fundamental Science Research Project for Chemistry & BiologyShaanxi Fundamental Science Research Project for Chemistry & BiologyGuangDong Basic and Applied Basic Research FoundationInnovation Foundation for Doctoral Dissertations of Northwestern Polytechnical UniversityInnovation Foundation for Doctoral Dissertations of Northwestern Polytechnical UniversityInnovation Foundation for Doctoral Dissertations of Northwestern Polytechnical UniversityPostdoctoral Research Foundation of ChinaPostdoctoral Research Foundation of China

31872804321016702024JC-YBMS-15122JHQ05422JHZ0072021A1515110341CX2021040CX2022079CX20230962021M6926442021M702674

2024

10.1111/jipb.13615

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(5)
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