PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

Xiaofeng Luo ¹Yujia Dai ¹Baoshan Xian ¹Jiahui Xu ²Ranran Zhang ²Muhammad Saad Rehmani ²Chuan Zheng ²Xiaoting Zhao ²Kaitao Mao ²Xiaotong Ren ²Shaowei Wei ¹Lei Wang ¹Juan He ¹Weiming Tan ³Junbo Du ⁴Weiguo Liu ⁴Shu Yuan ⁵Kai Shu¹

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作者信息

1. Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection,School of Ecology and Environment,Northwestern Polytechnical University,Xi'an 710129,China;Research & Development,Institute of Northwestern Polytechnical University in Shenzhen,Shenzhen 518057,China
2. Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection,School of Ecology and Environment,Northwestern Polytechnical University,Xi'an 710129,China
3. College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China
4. Institute of Ecological Agriculture,Sichuan Agricultural University,Chengdu 611130,China
5. College of Resources,Sichuan Agricultural University,Chengdu 611130,China
折叠

Abstract

Transcriptional regulation plays a key role in the control of seed dormancy,and many transcription factors(TFs)have been documented.However,the mechanisms underlying the interactions between different TFs within a transcriptional complex regu-lating seed dormancy remain largely unknown.Here,we showed that TF PHYTOCHROME-INTERACTING FACTOR4(PIF4)physically interacted with the ab-scisic acid(ABA)signaling responsive TF ABSCISIC ACID INSENSITIVE4(ABI4)to act as a transcriptional complex to promote ABA biosynthesis and signaling,finally deepening primary seed dormancy.Both pif4 and abi4 single mutants exhibited a decreased pri-mary seed dormancy phenotype,with a synergistic effect in the pif4/abi4 double mutant.PIF4 binds to ABI4 to form a heterodimer,and ABI4 stabilizes PIF4 at the protein level,whereas PIF4 does not affect the protein stabilization of ABI4.Subsequently,both TFs independently and synergistically promoted the ex-pression of ABI4 and NCED6,a key gene for ABA anabolism.The genetic evidence is also consistent with the phenotypic,physiological and biochemical analysis results.Altogether,this study revealed a transcriptional regulatory cascade in which the PIF4-ABI4 transcriptional activator complex synerg-istically enhanced seed dormancy by facilitating ABA biosynthesis and signaling.

Key words

ABA/ABI4/PIF4/seed dormancy and germination/transcriptional complex

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基金项目

National Natural Science Foundation of China(31872804)

National Natural Science Foundation of China(32101670)

Natural Science Basic Research Program of Shaanxi(2024JC-YBMS-151)

Shaanxi Fundamental Science Research Project for Chemistry & Biology(22JHQ054)

Shaanxi Fundamental Science Research Project for Chemistry & Biology(22JHZ007)

GuangDong Basic and Applied Basic Research Foundation(2021A1515110341)

Innovation Foundation for Doctoral Dissertations of Northwestern Polytechnical University(CX2021040)

Innovation Foundation for Doctoral Dissertations of Northwestern Polytechnical University(CX2022079)

Innovation Foundation for Doctoral Dissertations of Northwestern Polytechnical University(CX2023096)

Postdoctoral Research Foundation of China(2021M692644)

Postdoctoral Research Foundation of China(2021M702674)

出版年

2024

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCDCSCD

影响因子：0.921

ISSN：1672-9072

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