首页|Phase separation of S-RNase promotes self-incompatibility in Petunia hybrida∞
Phase separation of S-RNase promotes self-incompatibility in Petunia hybrida∞
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Self-incompatibility(SI)is an intraspecific re-productive barrier widely present in angiosperms.The SI system with the broadest occurrence in angiosperms is based on an S-RNase linked to a cluster of multiple S-locus F-box(SLF)genes found in the Solanaceae,Plantaginaceae,Rosaceae,and Rutaceae.Recent studies reveal that non-self S-RNase is degraded by the Skip Cullin F-box(SCF)SLF-mediated ubiquitin-proteasome system in a collaborative manner in Petunia,but how self-RNase functions largely remains mysterious.Here,we show that S-RNases form S-RNase con-densates(SRCs)in the self-pollen tube cytoplasm through phase separation and the disruption of SRC formation breaks SI in self-incompatible Petunia hybrida.We further find that the pistil SI factors of a small asparagine-rich protein HT-B and thioredoxin h together with a reduced state of the pollen tube all promote the expansion of SRCs,which then sequester several actin-binding pro-teins,including the actin polymerization factor PhABRACL,the actin polymerization activity of which is reduced by S-RNase in vitro.Meanwhile,we find that S-RNase variants lacking con-densation ability fail to recruit PhABRACL and are unable to induce actin foci formation required for pollen tube growth inhibition.Taken together,our results demonstrate that phase separation of S-RNase promotes SI response in P.hybrida,revealing a new mode of S-RNase action.
The State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,the Chinese Academy of Sciences,Beijing 100101,China
University of the Chinese Academy of Sciences,Beijing 100049,China
Beijing Institute of Genomics,Chinese Academy of Sciences,National Center for Bioinformation,Beijing 100101,China
National Natural Science Foundation of ChinaStrategic Priority Research Program of the Chinese Academy of Sciences
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