首页|A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors∞

A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors∞

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In plants,thousands of nucleus-encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC(translocon on the outer chloroplast membrane)and the TOM(translocon on the outer mitochondrial membrane)complexes for import into those organelles.The degradation pathways for these receptors are unclear.Here,we discovered a converged ubiquitin-proteasome pathway for the degradation of Arabidopsis thaliana TOC and TOM tail-anchored receptors.The receptors are ubiquiti-nated by E3 ligase(s)and pulled from the outer membranes by the AAA+adenosine triphosphatase CDC48,after which a previously uncharacterized cytosolic protein,transmembrane domain(TMD)-binding protein for tail-anchored outer membrane proteins(TTOP),binds to the exposed TMDs at the C termini of the receptors and CDC48,and delivers these complexes to the 26S proteasome.

26S proteasomeCDC48SP1TOCTOMubiquiti-nation

Meijing Yang、Shuai Chen、Shey-Li Lim、Lang Yang、Jia Yi Zhong、Koon Chuen Chan、Zhizhu Zhao、Kam-Bo Wong、Junqi Wang、Boon Leong Lim

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School of Biological Sciences,University of Hong Kong,Pokfulam 999077,Hong Kong,China

Department of Biology,Southern University of Science and Technology,Shenzhen 518055,China

School of Biomedical Engineering,Shenzhen University,Shenzhen 518060,China

School of Life Sciences,The Chinese University of Hong Kong,Shatin 999077,Hong Kong,China

State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong,Shatin 999077,Hong Kong,China

HKU Shenzhen Institute of Research and Innovation,Shenzhen 518052,China

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Science,Technology and Innovation Commission of Shenzhen Municipality(Basic Research Program)Hong Kong Research Grants Council Area of Excellence SchemeInnovation and Technology Fund(Funding Support to State Key Laboratory of Agrobiotechnology)of the Hong Kong Special Adminis

201708183000803AoE/M-403/16

2024

10.1111/jipb.13645

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(5)