Abstract
Leaves are the main photosynthesis organ that directly determines crop yield and biomass.Dis-secting the regulatory mechanism of leaf devel-opment is crucial for food security and ecosystem turn-over.Here,we identified the novel function of R2R3-MYB transcription factors CsRAXs in regu-lating cucumber leaf size and fruiting ability.Csrax5 single mutant exhibited enlarged leaf size and stem diameter,and Csrax1/2/5 triple mutant displayed further enlargement phenotype.Over-expression of CsRAX1 or CsRAX5 gave rise to smaller leaf and thinner stem.The fruiting ability of Csrax1/2/5 plants was significantly enhanced,while that of CsRAX5 overexpression lines was greatly weakened.Similarly,cell number and free auxin level were elevated in mutant plants while decreased in overexpression lines.Biochemical data indicated that CsRAX1/5 directly promoted the expression of auxin glucosyltransferase gene CsUGT74E2.Therefore,our data suggested that CsRAXs function as repressors for leaf size de-velopment by promoting auxin glycosylation to decrease free auxin level and cell division in cu-cumber.Our findings provide new gene targets for cucumber breeding with increased leaf size and crop yield.
基金项目
National Natural Science Foundation of China(32025033)
National Natural Science Foundation of China(31930097)
National Natural Science Foundation of China(32372699)
Pinduoduo-China Agricultural University Research Fund(PC2023B01002)
维普
万方数据