首页|CsRAXs negatively regulate leaf size and fruiting ability through auxin glycosylation in cucumber

CsRAXs negatively regulate leaf size and fruiting ability through auxin glycosylation in cucumber

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Leaves are the main photosynthesis organ that directly determines crop yield and biomass.Dis-secting the regulatory mechanism of leaf devel-opment is crucial for food security and ecosystem turn-over.Here,we identified the novel function of R2R3-MYB transcription factors CsRAXs in regu-lating cucumber leaf size and fruiting ability.Csrax5 single mutant exhibited enlarged leaf size and stem diameter,and Csrax1/2/5 triple mutant displayed further enlargement phenotype.Over-expression of CsRAX1 or CsRAX5 gave rise to smaller leaf and thinner stem.The fruiting ability of Csrax1/2/5 plants was significantly enhanced,while that of CsRAX5 overexpression lines was greatly weakened.Similarly,cell number and free auxin level were elevated in mutant plants while decreased in overexpression lines.Biochemical data indicated that CsRAX1/5 directly promoted the expression of auxin glucosyltransferase gene CsUGT74E2.Therefore,our data suggested that CsRAXs function as repressors for leaf size de-velopment by promoting auxin glycosylation to decrease free auxin level and cell division in cu-cumber.Our findings provide new gene targets for cucumber breeding with increased leaf size and crop yield.

auxin glycosylationCsRAXsCsUGT74E2fruiting abilityleaf size

Jiacai Chen、Liu Liu、Guangxin Chen、Shaoyun Wang、Ye Liu、Zeqin Zhang、Hongfei Li、Liming Wang、Zhaoyang Zhou、Jianyu Zhao、Xiaolan Zhang

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Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,Department of Vegetable Sciences,China Agricultural University,Beijing 100193,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaPinduoduo-China Agricultural University Research Fund

320250333193009732372699PC2023B01002

2024

10.1111/jipb.13655

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(5)
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