首页|Autophagy receptor ZmNBR1 promotes the autophagic degradation of ZmBRl1a and enhances drought tolerance in maize

Autophagy receptor ZmNBR1 promotes the autophagic degradation of ZmBRl1a and enhances drought tolerance in maize

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Drought stress is a crucial environmental factor that limits plant growth,development,and productivity.Autophagy of misfolded proteins can help alleviate the damage caused in plants experiencing drought.However,the mechanism of autophagy-mediated drought tolerance in plants remains largely unknown.Here,we cloned the gene for a maize(Zea mays)selective autophagy receptor,NEXT TO BRCA1 GENE 1(ZmNBR1),and identified its role in the re-sponse to drought stress.We observed that drought stress increased the accumulation of autophago-somes.RNA sequencing and reverse transcription-quantitative polymerase chain reaction showed that ZmNBR1 is markedly induced by drought stress.ZmNBR1 overexpression enhanced drought tolerance,while its knockdown reduced drought tol-erance in maize.Our results established that ZmNBR1 mediates the increase in autophagosomes and autophagic activity under drought stress.ZmNBR1 also affects the expression of genes related to autophagy under drought stress.Moreover,we determined that BRASSINOSTEROID INSENSITIVE 1A(ZmBRI1a),a brassinosteroid receptor of the BRI1-like family,interacts with ZmNBR1.Phenotype analysis showed that ZmBRI1a negatively regulates drought tolerance in maize,and genetic analysis in-dicated that ZmNBR1 acts upstream of ZmBRI1a in regulating drought tolerance.Furthermore,ZmNBR1 facilitates the autophagic degradation of ZmBRI1a under drought stress.Taken together,our results reveal that ZmNBR1 regulates the expression of autophagy-related genes,thereby increasing auto-phagic activity and promoting the autophagic deg-radation of ZmBRI1a under drought stress,thus en-hancing drought tolerance in maize.These findings provide new insights into the autophagy degradation of brassinosteroid signaling components by the au-tophagy receptor NBR1 under drought stress.

autophagy receptordrought toleranceZea maysZmBRI1aZmNBR1

Yang Xiang、Guangdong Li、Qian Li、Yingxue Niu、Yitian Pan、Yuan Cheng、Xiangli Bian、Chongyang Zhao、Yuanhong Wang、Aying Zhang

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College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China

State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,China

Sanya Institute of Nanjing Agricultural University,Nanjing Agricultural University,Sanya 572025,China

National Natural Science Foundation of ChinaNatural Science Foundation of Jiangsu ProvinceFundamental Research Funds for the Central UniversitiesChina Postdoctoral Science FoundationChina Postdoctoral Science FoundationJiangsu Funding Program for Excellent Postdoctoral TalentOpen Competition Mechanism to Select the Best Candidates Fund of Jiangsu ProvinceKey Research and Development Program of Ningxia Hui Autonomous RegionAchievement Transformation Fund Project of Hainan Research Institute of Nanjing Agricultural University

32201707BK20220999KYQN20230252021M7017392023T1603232022ZB330JBGS[2021]0122023BCF01009NAUSY-CG-YB07

2024

10.1111/jipb.13662

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(6)
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