首页|Knockout of miR396 genes increases seed size and yield in soybean

Knockout of miR396 genes increases seed size and yield in soybean

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Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal feed.miR396 genes have been shown to negatively regulate grain size in rice,but whether miR396 family members may function in a similar manner in soybean is un-known.Here,we generated eight soybean mu-tants harboring different combinations of ho-mozygous mutations in the six soybean miR396 genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhong-huang 302(ZH302).Four triple mutants(mir396aci,mir396acd,mir396adf,and mir396cdf),two quadruple mutants(mir396-abcd and mir396acfi),and two quintuple mu-tants(mir396abcdf and mir396bcdfi)were characterized.We found that plants of all the mir396 mutants produced larger seeds com-pared to ZH302 plants.Field tests showed that mir396adf and mir396cdf plants have sig-nificantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower latitude.In contrast,mir396abcdf and mir396bcdfi plants have increased plant height and decreased yield in growth zones with rela-tively high latitude due to lodging issues,but they are suited for low latitude growth zones with increased yield without lodging problems.Taken together,our study demonstrated that loss-of-function of miR396 genes leads to sig-nificantly enlarged seed size and increased yield in soybean,providing valuable germ-plasms for breeding high-yield soybean.

Cas12SF01CRISPR/CasmiR396seed sizesoy-beanyield

Hongtao Xie、Fei Su、Qingfeng Niu、Leping Geng、Xuesong Cao、Minglei Song、Jinsong Dong、Zai Zheng、Rui Guo、Yang Zhang、Yuanwei Deng、Zhanbo Ji、Kang Pang、Jian-Kang Zhu、Jianhua Zhu

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School of Life Sciences,Anhui Agricultural University,Hefei 230036,China

Research Center for Biological Breeding Technology,Research Institute of Frontier Science,Anhui Agricultural University,Hefei 230036,China

Bellagen Biotechnology Co.Ltd,Jinan 250000,China

Institute of Crop Sciences/National Nanfan Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Gene Editing Technologies(Hainan),Ministry of Agriculture and Rural Affairs,Sanya 572025,China

Southern University of Science,and Technology,Shenzhen 518055,China

Hainan Yazhou Bay Seed Laboratory,Sanya 572024,China

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Bellagen Biotechnology Inc.,and by the National Key Research and Development Program of ChinaSeed-Industrialized Development Program in Shandong Province,ChinaKey Research & Development Program of Shandong Province,ChinaInnovative research team of Anhui EducationNational Natural science Foundation of ChinaPhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City

NK20220103012021LZGC0032021LZGC0122022AH01005632270367HSPHDSRF-2023-11-007

2024

10.1111/jipb.13660

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(6)
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