The transcriptional control of LclDL1-LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission

Xingshuai Ma ¹Zidi He ¹Ye Yuan ²Zhijian Liang ¹Hang Zhang ¹Vilde Olsson Lalun ³Zhuoyi Liu ¹Yanqing Zhang ⁴Zhiqiang Huang ¹Yulian Huang ¹Jianguo Li ¹Minglei Zhao¹

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作者信息

1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangdong Laboratory for Lingnan Modern Agriculture,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China),Ministry of Agriculture and Rural Affairs,Guangdong Litchi Engineering Research Center,College of Horticulture,South China Agricultural University,Guangzhou 510642,China
2. Dongguan Botanical Garden,Dongguan 523128,China
3. Section for Genetics and Evolutionary Biology,Department of Biosciences,University of Oslo,Blindernveien 31,Oslo 0316,Norway
4. College of Agriculture,Guangxi University,Nanning 530004,China
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Abstract

At the physiological level,the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in plants.How-ever,the underlying molecular mechanisms remain unknown.Here,we identified transcription factors involved in indoleacetic acid(IAA)and ethylene(ET)signaling that directly regulate the expression of IN-FLORESCENCE DEFICIENT IN ABSCISSION(IDA)and its receptor HAESA(HAE),which are key com-ponents initiating abscission.Specifically,litchi IDA-like 1(LcIDL1)interacts with the receptor HAESA-like 2(LcHSL2).Through in vitro and in vivo experiments,we determined that the auxin response factor LcARF5 directly binds and activates both LcIDL1 and LcHSL2.Furthermore,we found that the ETHYLENE INSENSITIVE 3-like transcription factor LcEIL3 di-rectly binds and activates LcIDL1.The expression of IDA and HSL2 homologs was enhanced in LcARF5 and LcEIL3 transgenic Arabidopsis plants,but re-duced in ein3 eil1 mutants.Consistently,the ex-pressions of LcIDL1 and LcHSL2 were significantly decreased in LcARF5-and LcEIL3-silenced fruitlet abscission zones(FAZ),which correlated with a lower rate of fruitlet abscission.Depletion of auxin led to an increase in 1-aminocyclopropane-1-carboxylic acid(the precursor of ethylene)levels in the litchi FAZ,followed by abscission activation.Throughout this process,LcARF5 and LcEIL3 were induced in the FAZ.Collectively,our findings suggest that the mo-lecular interactions between litchi AUXIN RESPONSE FACTOR 5(LcARF5)-LcIDL1/LcHSL2 and LcEIL3-LclDL1 signaling modules play a role in regulating fruitlet abscission in litchi and provide a long-sought mechanistic explanation for how the interplay be-tween auxin and ethylene is translated into the mo-lecular events that initiate abscission.

Key words

auxin/ethylene/fruit abscission/IDA-HAE/HSL2/litchi

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基金项目

National Natural Science Foundation of China(32330092)

National Natural Science Foundation of China(32202447)

National Natural Science Foundation of China(32072544)

National Natural Science Foundation of China(32072514)

Natural Science Foundation of Guangdong Province,China(2021B1515120082)

Natural Science Foundation of Guangdong Province,China(2023A1515012661)

China Postdoctoral Science Foundation(2022M721209)

Laboratory of Lingnan Modern Agriculture Project(NZ NT2021004)

Guangdong Rural Revitalization Strategic Foundation(20211800400072)

出版年

2024

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

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