A transcriptional cascade mediated by two APETALA2 family members orchestrates carotenoid biosynthesis in tomato

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外文摘要：Carotenoids are important nutrients for human health that must be obtained from plants since they cannot be biosynthesized by the human body.Dissecting the regulatory mechanism of carotenoid metabolism in plants represents the first step toward manipu-lating carotenoid contents in plants by molecular design breeding.In this study,we determined that SIAP2c,an APETALA2(AP2)family member,acts as a transcriptional repressor to regulate carotenoid bi-osynthesis in tomato(Solanum lycopersicum).Knockout of SIAP2c in both the"MicroTom"and"Ailsa Craig"backgrounds resulted in greater lycopene accumulation,whereas overexpression of this gene led to orange-ripe fruit with significantly lower lycopene contents than the wild type.We es-tablished that SIAP2c represses the expression of genes involved in lycopene biosynthesis by directly binding to the cis-elements in their promoters.Moreover,SIAP2c relies on its EAR motif to recruit the co-repressors TOPLESS(TPL)2/4 and forms a complex with histone deacetylase(had)1/3,thereby reducing the histone acetylation levels of lycopene biosynthesis genes.Furthermore,SIAP2a,a homolog of SIAP2c,acts upstream of SIAP2c and alleviates the SIAP2c-induced repression of lycopene biosyn-thesis genes by inhibiting SIAP2c transcription during fruit ripening.Therefore,we identified a transcrip-tional cascade mediated by AP2 family members that regulates lycopene biosynthesis during fruit ripening in tomato,laying the foundation for the manipulation of carotenoid metabolism in plants.

外文关键词：

APETALA2 family memberscarotenoid biosynthesistomatotranscriptional cascade

作者：

Xiaoqing He、Kaidong Liu、Yi Wu、Weijie Xu、Ruochen Wang、Julien Pirrello、Mondher Bouzayen、Mengbo Wu、Mingchun Liu

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作者单位：

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China

Life Science and Technology School,Lingnan Normal University,Zhanjiang 524048,China

Laboratoire de Recherche en Sciences Végétales-Génomique et Biotechnologie des Fruits-UMR5546,Université de Toulouse,CNRS,UPS,Toulouse-INP,Toulouse 31013,France

基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaApplied Basic Research Category of Science and Technology Program of Sichuan ProvinceApplied Basic Research Category of Science and Technology Program of Sichuan ProvinceApplied Basic Research Category of Science and Technology Program of Sichuan ProvinceTechnology Innovation and Application Development Program of ChongqingNatural Science Foundation of Sichuan Province,ChinaInstitutional Research Funding of Sichuan University

项目编号：

32372780321726432021YFQ00712022YFSY0059-12021YFYZ0010-5-LHcstc2021jscxcylhX00012023NSFSC19912022SCUNL105

出版年：

2024

DOI：

10.1111/jipb.13650

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(6)