A transcriptional cascade mediated by two APETALA2 family members orchestrates carotenoid biosynthesis in tomato

Xiaoqing He ¹Kaidong Liu ²Yi Wu ¹Weijie Xu ¹Ruochen Wang ¹Julien Pirrello ³Mondher Bouzayen ³Mengbo Wu ¹Mingchun Liu¹

扫码查看

作者信息

1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China
2. Life Science and Technology School,Lingnan Normal University,Zhanjiang 524048,China
3. Laboratoire de Recherche en Sciences Végétales-Génomique et Biotechnologie des Fruits-UMR5546,Université de Toulouse,CNRS,UPS,Toulouse-INP,Toulouse 31013,France
折叠

Abstract

Carotenoids are important nutrients for human health that must be obtained from plants since they cannot be biosynthesized by the human body.Dissecting the regulatory mechanism of carotenoid metabolism in plants represents the first step toward manipu-lating carotenoid contents in plants by molecular design breeding.In this study,we determined that SIAP2c,an APETALA2(AP2)family member,acts as a transcriptional repressor to regulate carotenoid bi-osynthesis in tomato(Solanum lycopersicum).Knockout of SIAP2c in both the"MicroTom"and"Ailsa Craig"backgrounds resulted in greater lycopene accumulation,whereas overexpression of this gene led to orange-ripe fruit with significantly lower lycopene contents than the wild type.We es-tablished that SIAP2c represses the expression of genes involved in lycopene biosynthesis by directly binding to the cis-elements in their promoters.Moreover,SIAP2c relies on its EAR motif to recruit the co-repressors TOPLESS(TPL)2/4 and forms a complex with histone deacetylase(had)1/3,thereby reducing the histone acetylation levels of lycopene biosynthesis genes.Furthermore,SIAP2a,a homolog of SIAP2c,acts upstream of SIAP2c and alleviates the SIAP2c-induced repression of lycopene biosyn-thesis genes by inhibiting SIAP2c transcription during fruit ripening.Therefore,we identified a transcrip-tional cascade mediated by AP2 family members that regulates lycopene biosynthesis during fruit ripening in tomato,laying the foundation for the manipulation of carotenoid metabolism in plants.

Key words

APETALA2 family members/carotenoid biosynthesis/tomato/transcriptional cascade

引用本文复制引用

基金项目

National Natural Science Foundation of China(32372780)

National Natural Science Foundation of China(32172643)

Applied Basic Research Category of Science and Technology Program of Sichuan Province(2021YFQ0071)

Applied Basic Research Category of Science and Technology Program of Sichuan Province(2022YFSY0059-1)

Applied Basic Research Category of Science and Technology Program of Sichuan Province(2021YFYZ0010-5-LH)

Technology Innovation and Application Development Program of Chongqing(cstc2021jscxcylhX0001)

Natural Science Foundation of Sichuan Province,China(2023NSFSC1991)

Institutional Research Funding of Sichuan University(2022SCUNL105)

出版年

2024

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

段落导航