首页|TabHLH27 orchestrates root growth and drought tolerance to enhance water use efficiency in wheat

TabHLH27 orchestrates root growth and drought tolerance to enhance water use efficiency in wheat

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Cultivating high-yield wheat under limited water resources is crucial for sustainable agriculture in semiarid regions.Amid water scarcity,plants ac-tivate drought response signaling,yet the delicate balance between drought tolerance and develop-ment remains unclear.Through genome-wide as-sociation studies and transcriptome profiling,we identified a wheat atypical basic helix-loop-helix(bHLH)transcription factor(TF),TabHLH27-A1,as a promising quantitative trait locus candidate for both relative root dry weight and spikelet number per spike in wheat.TabHLH27-A1/B1/D1 knock-out reduced wheat drought tolerance,yield,and water use efficiency(WUE).TabHLH27-A1 ex-hibited rapid induction with polyethylene glycol(PEG)treatment,gradually declining over days.It activated stress response genes such as TaCBL8-B1 and TaCPI2-A1 while inhibiting root growth genes like TaSH15-B1 and TaWRKY70-B1 under short-term PEG stimulus.The distinct transcrip-tional regulation of TabHLH27-A1 involved diverse interacting factors such as TaABI3-D1 and TabZIP62-D1.Natural variations of TabHLH27-A1 influence its transcriptional responses to drought stress,with TabHLH27AA1Hap-Ⅱ associated with stronger drought tolerance,larger root system,more spikelets,and higher WUE in wheat.Sig-nificantly,the excellent TabHLH27-A1Hap-Ⅱ was selected during the breeding process in China,and introgression of TabHLH27-A1Hap-Ⅱ allele im-proved drought tolerance and grain yield,espe-cially under water-limited conditions.Our study highlights TabHLH27-A1's role in balancing root growth and drought tolerance,providing a genetic manipulation locus for enhancing WUE in wheat.

drought toleranceGWASroot growthwheatWUE

Dongzhi Wang、Xiuxiu Zhang、Yuan Cao、Aamana Batool、Yonaxin Xu、Yunzhou Qiao、Yongpeng Li、Hao Wang、Xuelei Lin、Xiaomin Bie、Xiansheng Zhang、Ruilian Jing、Baodi Dong、Yiping Tong、Wan Teng、Xigang Liu、Jun Xiao

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Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China

University of Chinese Academy of Sciences,Beijing 100049,China

Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050022,China

Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai'an 271018,China

State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Ministry of Education Key Laboratory of Molecular and Cellular Biology,Hebei Research Center of the Basic Discipline of Cell Biology,Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation,Hebei Key Laboratory of Molecular and Cellular Biology,College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China

Centre of Excellence for Plant and Microbial Science(CEPAMS),JIC-CAS,Beijing 100101,China

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Strategic Priority Research Program of the Chinese Academy of SciencesNational Key Research and Development Program of ChinaHebei Natural science FoundationFulltime introduction of highend talent research projectNational Natural Sciences Foundation of ChinaNational Natural Sciences Foundation of ChinaNational Natural Sciences Foundation of ChinaBeijing Natural Science Foundation Outstanding Youth ProjectMajor Basic Research Program of Shandong Natural Science FoundationSeed Science and Technology Innovation Team Project of Shijiazhuang

XDA240102042021YFD1201500C20212050132020HBQZYC00432100492U22A600932072004JQ23026ZR2019ZD15232490472A

2024

10.1111/jipb.13670

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(7)
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