The miR159a-DUO1 module regulates pollen development by modulating auxin biosynthesis and starch metabolism in citrus

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外文摘要：Achieving seedlessness in citrus varieties is one of the important objectives of citrus breeding.Male sterility associated with abnormal pollen develop-ment is an important factor in seedlessness.How-ever,our understanding of the regulatory mecha-nism underlying the seedlessness phenotype in citrus is still limited.Here,we determined that the miR159a-DUO1 module played an important role in regulating pollen development in citrus,which fur-ther indirectly modulated seed development and fruit size.Both the overexpression of csi-miR159a and the knocking out of DUO1 in Hong Kong kumquat(Fortunella hindsii)resulted in small and seedless fruit phenotypes.Moreover,pollen was severely aborted in both transgenic lines,with ar-rested pollen mitotic I and abnormal pollen starch metabolism.Through additional cross-pollination experiments,DUO1 was proven to be the key target gene for miR159a to regulate male sterility in citrus.Based on DNA affinity purification se-quencing(DAP-seq),RNA-seq,and verified inter-action assays,YUC2/YUC6,SS4 and STP8 were identified as downstream target genes of DUO1,those were all positively regulated by DUO1.In transgenic F.hindsii lines,the miR159a-DUO1 module down-regulated the expression of YUC2I YUC6,which decreased indoleacetic acid(IAA)levels and modulated auxin signaling to repress pollen mitotic I.The miR159a-DUO1 module re-duced the expression of the starch synthesis gene SS4 and sugar transport gene STP8 to disrupt starch metabolism in pollen.Overall,this work re-veals a new mechanism by which the miR159a-DUO1 module regulates pollen development and elucidates the molecular regulatory network un-derlying male sterility in citrus.

外文关键词：

auxincitrusmale sterilitymiRNApollen develop-mentseedlessnessstarch metabolism

作者：

Yanhui Xu、Wenxiu Tian、Minqiang Yin、Zhenmei Cai、Li Zhang、Deyi Yuan、Hualin Yi、Juxun Wu

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作者单位：

National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,Huazhong Agricultural University,Wuhan 430070,China

Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of the Ministry of Education and Key Laboratory of Non-Wood Forest Products of the Forestry Ministry,Central South University of Forestry and Technology,Changsha 410004,China

基金：

National Natural Science Foundation of China(NSFC)National Natural Science Foundation of China(NSFC)National Modern Citrus Industry SystemHubei Province Science and Technology Plan ProjectHubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees(Institute of Fruit and Tea,Hubei Academy of Agricult

项目编号：

3207254131601729CARS-262023BEB025GSSZ202302

出版年：

2024

DOI：

10.1111/jipb.13656

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(7)