Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations

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外文摘要：The heading date of rice is a crucial agronomic characteristic that influences its adaptability to dif-ferent regions and its productivity potential.Despite the involvement of WRKY transcription factors in various biological processes related to develop-ment,the precise mechanisms through which these transcription factors regulate the heading date in rice have not been well elucidated.The present study identified OsWRKY11 as a WRKY tran-scription factor which exhibits a pivotal function in the regulation of the heading date in rice through a comprehensive screening of a clustered regularly interspaced palindromic repeats(CRISPR)-CRISPR-associated nuclease 9 mutant library that specifically targets the WRKY genes in rice.The heading date of oswrky11 mutant plants and OsWRKY11-overexpressing plants was delayed compared with that of the wild-type plants under short-day and long-day conditions.Mechanistic investigation revealed that OsWRKY11 exerts dual effects on transcriptional promotion and sup-pression through direct and indirect DNA binding,respectively.Under normal conditions,OsWRKY11 facilitates flowering by directly inducing the ex-pression of OsMADS14 and OsMADS15.The pres-ence of elevated levels of OsWRKY11 protein pro-mote formation of a ternary protein complex involving OsWRKY11,Heading date 1(Hd1),and Days to heading date 8(DTH8),and this complex then suppresses the expression of Ehd1,which leads to a delay in the heading date.Subsequent investigation revealed that a mild drought condition resulted in a modest increase in OsWRKY11 expression,promoting heading.Conversely,under severe drought conditions,a significant upregula-tion of OsWRKY11 led to the suppression of Ehd1 expression,ultimately causing a delay in heading date.Our findings uncover a previously un-acknowledged mechanism through which the tran-scription factor OsWRKY11 exerts a dual impact on the heading date by directly and indirectly binding to the promoters of target genes.

外文关键词：

heading dateOsWRKY11ricetranscription factors

作者：

Lirong Zhao、Yunwei Liu、Yi Zhu、Shidie Chen、Yang Du、Luyao Deng、Lei Liu、Xia Li、Wanqin Chen、Zhiyu Xu、Yangyang Xiong、You Ming、Siyu Fang、Ligang Chen、Houping Wang、Diqiu Yu

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作者单位：

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Mengla 666303,China

College of Life Sciences,University of Chinese Academy of Sciences,Beijing 101408,China

State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China

School of Life Sciences,Yunnan University,Kunming 650500,China

Southwest United Graduate School,Yunnan University,Kunming 650092,China

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基金：

Natural Science Foundation of ChinaNatural Science Foundation of ChinaNatural Science Foundation of ChinaNatural Science Foundation of ChinaYunnan Applied Basic Research ProjectsYunnan Applied Basic Research ProjectsYunnan Applied Basic Research ProjectsYunnan Applied Basic Research ProjectsYunnan Applied Basic Research ProjectsYunnan High Level Talents Special Support PlanScientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan UniversityScientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan UniversityScientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University

项目编号：

32270356321600803207054532372030202201AV070002202401BC070005202301AT070111202201BF070001-019202001AW070009KC-22221687KC-23236546KC-23236419

出版年：

2024

DOI：

10.1111/jipb.13679

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(7)

参考文献量6