Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations

Lirong Zhao ¹Yunwei Liu ²Yi Zhu ³Shidie Chen ⁴Yang Du ²Luyao Deng ²Lei Liu ¹Xia Li ⁴Wanqin Chen ²Zhiyu Xu ²Yangyang Xiong ³You Ming ³Siyu Fang ²Ligang Chen ⁵Houping Wang ³Diqiu Yu⁶

扫码查看

作者信息

1. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Mengla 666303,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 101408,China
2. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China
3. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China;School of Life Sciences,Yunnan University,Kunming 650500,China
4. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China;Southwest United Graduate School,Yunnan University,Kunming 650092,China
5. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Mengla 666303,China
6. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China;School of Life Sciences,Yunnan University,Kunming 650500,China;Southwest United Graduate School,Yunnan University,Kunming 650092,China
折叠

Abstract

The heading date of rice is a crucial agronomic characteristic that influences its adaptability to dif-ferent regions and its productivity potential.Despite the involvement of WRKY transcription factors in various biological processes related to develop-ment,the precise mechanisms through which these transcription factors regulate the heading date in rice have not been well elucidated.The present study identified OsWRKY11 as a WRKY tran-scription factor which exhibits a pivotal function in the regulation of the heading date in rice through a comprehensive screening of a clustered regularly interspaced palindromic repeats(CRISPR)-CRISPR-associated nuclease 9 mutant library that specifically targets the WRKY genes in rice.The heading date of oswrky11 mutant plants and OsWRKY11-overexpressing plants was delayed compared with that of the wild-type plants under short-day and long-day conditions.Mechanistic investigation revealed that OsWRKY11 exerts dual effects on transcriptional promotion and sup-pression through direct and indirect DNA binding,respectively.Under normal conditions,OsWRKY11 facilitates flowering by directly inducing the ex-pression of OsMADS14 and OsMADS15.The pres-ence of elevated levels of OsWRKY11 protein pro-mote formation of a ternary protein complex involving OsWRKY11,Heading date 1(Hd1),and Days to heading date 8(DTH8),and this complex then suppresses the expression of Ehd1,which leads to a delay in the heading date.Subsequent investigation revealed that a mild drought condition resulted in a modest increase in OsWRKY11 expression,promoting heading.Conversely,under severe drought conditions,a significant upregula-tion of OsWRKY11 led to the suppression of Ehd1 expression,ultimately causing a delay in heading date.Our findings uncover a previously un-acknowledged mechanism through which the tran-scription factor OsWRKY11 exerts a dual impact on the heading date by directly and indirectly binding to the promoters of target genes.

Key words

heading date/OsWRKY11/rice/transcription factors

引用本文复制引用

基金项目

Natural Science Foundation of China(32270356)

Natural Science Foundation of China(32160080)

Natural Science Foundation of China(32070545)

Natural Science Foundation of China(32372030)

Yunnan Applied Basic Research Projects(202201AV070002)

Yunnan Applied Basic Research Projects(202401BC070005)

Yunnan Applied Basic Research Projects(202301AT070111)

Yunnan Applied Basic Research Projects(202201BF070001-019)

Yunnan Applied Basic Research Projects(202001AW070009)

Yunnan High Level Talents Special Support Plan()

Scientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University(KC-22221687)

Scientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University(KC-23236546)

Scientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University(KC-23236419)

出版年

2024

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

参考文献量6

段落导航