植物学报(英文版)2024,Vol.66Issue(7) :1385-1407.DOI:10.1111/jipb.13679

Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations

Lirong Zhao Yunwei Liu Yi Zhu Shidie Chen Yang Du Luyao Deng Lei Liu Xia Li Wanqin Chen Zhiyu Xu Yangyang Xiong You Ming Siyu Fang Ligang Chen Houping Wang Diqiu Yu
植物学报(英文版)2024,Vol.66Issue(7) :1385-1407.DOI:10.1111/jipb.13679

Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations

Lirong Zhao 1Yunwei Liu 2Yi Zhu 3Shidie Chen 4Yang Du 2Luyao Deng 2Lei Liu 1Xia Li 4Wanqin Chen 2Zhiyu Xu 2Yangyang Xiong 3You Ming 3Siyu Fang 2Ligang Chen 5Houping Wang 3Diqiu Yu6
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作者信息

  • 1. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Mengla 666303,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 101408,China
  • 2. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China
  • 3. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China;School of Life Sciences,Yunnan University,Kunming 650500,China
  • 4. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China;Southwest United Graduate School,Yunnan University,Kunming 650092,China
  • 5. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Mengla 666303,China
  • 6. State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan,Yunnan University,Kunming 650500,China;School of Life Sciences,Yunnan University,Kunming 650500,China;Southwest United Graduate School,Yunnan University,Kunming 650092,China
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Abstract

The heading date of rice is a crucial agronomic characteristic that influences its adaptability to dif-ferent regions and its productivity potential.Despite the involvement of WRKY transcription factors in various biological processes related to develop-ment,the precise mechanisms through which these transcription factors regulate the heading date in rice have not been well elucidated.The present study identified OsWRKY11 as a WRKY tran-scription factor which exhibits a pivotal function in the regulation of the heading date in rice through a comprehensive screening of a clustered regularly interspaced palindromic repeats(CRISPR)-CRISPR-associated nuclease 9 mutant library that specifically targets the WRKY genes in rice.The heading date of oswrky11 mutant plants and OsWRKY11-overexpressing plants was delayed compared with that of the wild-type plants under short-day and long-day conditions.Mechanistic investigation revealed that OsWRKY11 exerts dual effects on transcriptional promotion and sup-pression through direct and indirect DNA binding,respectively.Under normal conditions,OsWRKY11 facilitates flowering by directly inducing the ex-pression of OsMADS14 and OsMADS15.The pres-ence of elevated levels of OsWRKY11 protein pro-mote formation of a ternary protein complex involving OsWRKY11,Heading date 1(Hd1),and Days to heading date 8(DTH8),and this complex then suppresses the expression of Ehd1,which leads to a delay in the heading date.Subsequent investigation revealed that a mild drought condition resulted in a modest increase in OsWRKY11 expression,promoting heading.Conversely,under severe drought conditions,a significant upregula-tion of OsWRKY11 led to the suppression of Ehd1 expression,ultimately causing a delay in heading date.Our findings uncover a previously un-acknowledged mechanism through which the tran-scription factor OsWRKY11 exerts a dual impact on the heading date by directly and indirectly binding to the promoters of target genes.

Key words

heading date/OsWRKY11/rice/transcription factors

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基金项目

Natural Science Foundation of China(32270356)

Natural Science Foundation of China(32160080)

Natural Science Foundation of China(32070545)

Natural Science Foundation of China(32372030)

Yunnan Applied Basic Research Projects(202201AV070002)

Yunnan Applied Basic Research Projects(202401BC070005)

Yunnan Applied Basic Research Projects(202301AT070111)

Yunnan Applied Basic Research Projects(202201BF070001-019)

Yunnan Applied Basic Research Projects(202001AW070009)

Yunnan High Level Talents Special Support Plan()

Scientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University(KC-22221687)

Scientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University(KC-23236546)

Scientific Research and Innovation Project of Postgraduate Students in the Academic Degree of YunNan University(KC-23236419)

出版年

2024
植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子:0.921
ISSN:1672-9072
参考文献量6
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