Natural variation in MORE GRAINS 1 regulates grain number and grain weight in rice∞

Yingchun Han ¹Qianfeng Hu ²Nuo Gong ¹Huimin Yan ¹Najeeb Ullah Khan ²Yanxiu Du ¹Hongzheng Sun ¹Quanzhi Zhao ³Wanxi Peng ⁴Zichao Li ²Zhanying Zhang ²Junzhou Li¹

扫码查看

作者信息

1. Henan Key Laboratory of Rice Molecular Breeding and High Efficiency Production,College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China
2. Beijing Key Laboratory of Crop Genetic Improvement,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China
3. Henan Key Laboratory of Rice Molecular Breeding and High Efficiency Production,College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;Rice Industrial Technology Research Institute,Guizhou University,Guiyang 550025,China
4. School of Forestry,Henan Agricultural University,Zhengzhou 450046,China
折叠

Abstract

Grain yield is determined mainly by grain number and grain weight.In this study,we identified and characterized MORE GRAINS1(MOG1),a gene associated with grain number and grain weight in rice(Oryza sativa L.),through map-based cloning.Overexpression of MOG1 increased grain yield by 18.6％-22.3％under field conditions.We de-termined that MOG1,a bHLH transcription factor,interacts with OsbHLH107 and directly activates the expression of LONELY GUY(LOG),which encodes a cytokinin-activating enzyme and the cell expansion gene EXPANSIN-LIKE1(EXPLA1),positively regu-lating grain number per panicle and grain weight.Natural variations in the promoter and coding re-gions of MOG1 between Hap-LNW and Hap-HNW alleles resulted in changes in MOG1 expression level and transcriptional activation,leading to functional differences.Haplotype analysis revealed that Hap-HNW,which results in a greater number and heavier grains,has undergone strong selection but has been poorly utilized in modem lowland rice breeding.In summary,the MOG1-OsbHLH107 complex acti-vates LOG and EXPLA1 expression to promote cell expansion and division of young panicles through the cytokinin pathway,thereby increasing grain number and grain weight.These findings suggest that Hap-HNW could be used in strategies to breed high-yielding temperate japonica lowland rice.

Key words

grain number/grain weight/MOG1/natural variation/rice

引用本文复制引用

基金项目

STI2030 Major Projects(2023ZD0406803)

AgroST Project(NK2022050103)

National Natural Science Foundation of China(32372094)

National Natural Science Foundation of China(32272123)

National Natural Science Foundation of China(32072036)

Modern Agricultural Industry Technology System Projects of Henan,China(HARS-22-03-G3)

Key R&D projects of Henan(231111110500)

Key Laboratory of Functional Agriculture,Guizhou Province([2023]007)

Key Laboratory of Molecular Breeding for Grain and Oil crops,Guizhou Province([2023]008)

China Postdoctoral Science Foundation(2021M701108)

出版年

2024

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

参考文献量1

段落导航