Abstract
Vicinal oxygen chelate(VOC)proteins are members of an enzyme superfamily with dioxygenase or non-dioxygenase activities.However,the biological functions of VOC proteins in plants are poorly un-derstood.Here,we show that a VOC in Nicotiana benthamiana(NbVOC1)facilitates viral infection.NbVOC1 was significantly induced by infection by beet necrotic yellow vein virus(BNYW).Transient overexpression of NbVOC1 or its homolog from Beta vulgaris(BvVOC11)enhanced BNYW infection in N.benthamiana,which required the nuclear localization of VOC1.Consistent with this result,overexpressing NbVOC1 facilitated BNYW in-fection,whereas,knockdown and knockout of NbVOC1 inhibited BNYW infection in transgenic N.benthamiana plants.NbVOC1 interacts with the basic leucine zipper transcription factors bZIP17/28,which enhances their self-interaction and DNA binding to the promoters of unfolded protein re-sponse(UPR)-related genes.We propose that bZIP17/28 directly binds to the NbVOC1 promoter and induces its transcription,forming a positive feedback loop to induce the UPR and facilitating BNYW infection.Collectively,our results demon-strate that NbVOC1 positively regulates the UPR that enhances viral infection in plants.
基金项目
National Natural Science Foundation of China(32270165)
National Natural Science Foundation of China(31872921)
China Agricultural Industry Technology System(CARS-170304)
维普
万方数据