首页|AtRKD5 inhibits the parthenogenic potential mediated by AtBBM

AtRKD5 inhibits the parthenogenic potential mediated by AtBBM

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Parthenogenesis,the development of unfertilized egg cells into embryos,is a key component of apomixis.AtBBM(BABY BOOM),a crucial regulator of embryogenesis in Arabidopsis,possesses the capacity to shift nutritional growth toward re-productive growth.However,the mechanisms un-derlying AtBBM-induced parthenogenesis remain largely unexplored in dicot plants.Our findings re-vealed that in order to uphold the order of sexual reproduction,the embryo-specific promoter activity of AtBBM as well as repressors that inhibit its ex-pression in egg cells combine to limiting its ability to induce parthenogenesis.Notably,AtRKD5,a RWP-RK domain-containing(RKD)transcription factor,binds to the 3'end of AtBBM and is identified as one of the inhibitory factors for AtBBM expression in the egg cell.In the atrkd5 mutant,we success-fully achieved enhanced ectopic expression of AtBBM in egg cells,resulting in the generation of haploid offspring via parthenogenesis at a rate of 0.28％.Furthermore,by introducing chimeric Arabidopsis and rice BBM genes into the egg cell,we achieved a significant 4.6-fold enhance-ment in haploid induction through the atdmp8/9 mutant.These findings lay a strong foundation for further exploration of the BBM-mediated partheno-genesis mechanism and the improvement of hap-loid breeding efficiency mediated by the dmp8/9 mutant.

AtBBMatdmpAtRKD5haploid inductionparthe-nogenesis

Qiyan Liu、Dongfen Han、Denghu Cheng、Jinfan Chen、Shujuan Tian、Jiafa Wang、Man Liu、Li Yuan

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State Key Laboratory of Crop Stress Resistance and High-Efficiency Production,College of Horticulture,Northwest A&F University,Yangling 712100,China

Key-Area R&D Program of Guangdong ProvinceNational Youth Talent ProgramKey R&D Program of Shaanxi provinceNatural Science Foundation of Shaanxi Province

2022B0202060001A2790218012023-YBNY-0082022JM-112

2024

10.1111/jipb.13678

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(7)
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