首页|Overexpression of tonoplast Ca2+-ATPase in guard cells synergistically enhances stomatal opening and drought tolerance

Overexpression of tonoplast Ca2+-ATPase in guard cells synergistically enhances stomatal opening and drought tolerance

扫码查看
原文链接
  • 万方数据
Stomata play a crucial role in plants by controlling water status and responding to drought stress.However,simultaneously improving stomatai opening and drought tolerance has proven to be a significant challenge.To address this issue,we em-ployed the OnGuard quantitative model,which ac-curately represents the mechanics and coordination of ion transporters in guard cells.With the guidance of OnGuard,we successfully engineered plants that overexpressed the main tonoplast Ca2+-ATPase gene,ACA11,which promotes stomatai opening and enhances plant growth.Surprisingly,these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their stomata.Again,OnGuard assisted us in under-standing the mechanism behind the unexpected stomatal behaviors observed in the ACA11 over-expressing plants.Our study revealed that the over-expression of ACA11 facilitated the accumulation of Ca2+in the vacuole,thereby influencing Ca2+storage and leading to an enhanced Ca2+elevation in response to abscisic acid.This regulatory cascade finely tunes stomatal responses,ultimately leading to enhanced drought tolerance.Our findings under-score the importance of tonoplast Ca2+-ATPase in manipulating stomatal behavior and improving drought tolerance.Furthermore,these results high-light the diverse functions of tonoplast-localized ACA11 in response to different conditions,empha-sizing its potential for future applications in plant enhancement.

ACA11droughtguard cellsmodelingstomata

Jinghan Su、Bingqing He、Peiyuan Li、Baiyang Yu、Qiwen Cen、Lingfeng Xia、Yi Jing、Feibo Wu、Rucha Kamik、Dawei Xue、Michael R.Blatt、Yizhou Wang

展开 >

Institute of Crop Science,Zhejiang University,Hangzhou 310058,China

Laboratory of Plant Physiology and Biophysics,University of Glasgow,Glasgow G12 8QQ,UK

BGI Research,Sanya 572025,China

College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China

Zhejiang Provincial Key Laboratory of Crop Germplasm,Zhejiang University,Hangzhou 310058,China

展开 >

2024

10.1111/jipb.13721

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(8)