首页|The MADS-box transcription factor GmFULc promotes GmZTL4 gene transcription to modulate maturity in soybean

The MADS-box transcription factor GmFULc promotes GmZTL4 gene transcription to modulate maturity in soybean

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Flowering time and maturity are crucial agronomic traits that affect the regional adaptability of soy-bean plants.The development of soybean cultivars with early maturity adapted to longer days and colder climates of high latitudes is very important for ensuring normal ripening before frost begins.FUL belongs to the MADS-box transcription factor family and has several duplicated members in soybeans.In this study,we observed that over-expression of GmFULc in the Dongnong 50 cultivar promoted soybean maturity,while GmFULc knockout mutants exhibited late maturity.Chro-matin immunoprecipitation sequencing(ChIP-seq)and RNA sequencing(RNA-seq)revealed that GmFULc could bind to the CArG,bHLH and ho-meobox motifs.Further investigation revealed that GmFULc could directly bind to the CArG motif in the promoters of the GmZTL3 and GmZTL4 genes.Overexpression of GmZTL4 promoted soybean maturity,whereas the ztl4 mutants exhibited de-layed maturity.Moreover,we found that the cis el-ement box 4 motif of the GmZTL4 promoter,a motif of light response elements,played an important role in controlling the growth period.Deletion of this motif shortened the growth period by increasing the expression levels of GmZTL4.Functional inves-tigations revealed that short-day treatment pro-moted the binding of GmFULc to the promoter of GmZTL4 and inhibited the expression of E1 and E1Lb,ultimately resulting in the promotion of flow-ering and early maturation.Taken together,these findings suggest a novel photoperiod regulatory pathway in which GmFULc directly activates GmZTL4 to promote earlier maturity in soybean.

flowering timeGmFULcGmZTL4maturityphoto-period

Jingzhe Sun、Yucheng Liu、Yuhong Zheng、Yongguo Xue、Yuhuan Fan、Xiaofei Ma、Yujia Ji、Gaoyuan Liu、Xiaoming Zhang、Yang Li、Shuming Wang、Zhixi Tian、Lin Zhao

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Key Laboratory of Soybean Biology of Ministry of Education China,Northeast Agricultural University,Harbin 150030,China

Qingdao Institute of Bioenergy and Bioprocess Technology,The Chinese Academy of Sciences,Qingdao 266101,China

State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,The Chinese Academy of Sciences,Beijing 100101,China

Jilin Academy of Agricultural Sciences(China Agricultural Science and Technology Northeast Inno-vation Center),Changchun 130033,China

Institute of Soybean Research,Heilongjiang Provincial Academy of Agricultural Sciences,Harbin 150086,China

Depatment of Environmental and Plant Biology,Ohio University,Athens,Ohio 45701,USA

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2024

10.1111/jipb.13682

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(8)