GmDFB1,an ARM-repeat superfamily protein,regulates floral organ identity through repressing siRNA-and miRNA-mediated gene silencing in soybean

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外文摘要：The development of flowers in soybean(Glycine max)is essential for determining the yield potential of the plant.Gene silencing pathways are involved in modulating flower development,but their full elucidation is still incomplete.Here,we conducted a forward genetic screen and identified an abnormal flower mutant,deformed floral bud1-1(Gmdfb1-1),in soybean.We mapped and identified the causal gene,which encodes a member of the armadillo(ARM)-repeat superfamily.Using small RNA se-quencing(sRNA-seq),we found an abnormal ac-cumulation of small interfering RNAs(siRNAs)and microRNA(miRNAs)in the Gmdfb1 mutants.We further demonstrated that GmDFB1 interacts with the RNA exosome cofactor SUPER KILLER7(GmSKI7).Additionally,GmDFB1 interacts with the PIWI domain of ARGONAUTE1(GmAGO1)to inhibit the cleavage efficiency on the target genes of sRNAs.The enhanced gene silencing mediated by siRNA and miRNA in the Gmdfb1 mutants leads to the downregulation of their target genes associated with flower development.This study revealed the crucial role of GmDFB1 in regulating floral organ identity in soybean probably by participating in two distinct gene silencing pathways.

外文关键词：

ARM-repeat superfamily proteinARGONAUTE 1SUPER KILLER 7flower developmentgene silencingsmall RNAssoybean

作者：

Jie Li、Wenxiao Zhang、Qing Lu、Jiaqi Sun、Chuang Cheng、Shiyu Huang、Shuo Li、Qiang Li、Wei Zhang、Chuanen Zhou、Bin Liu、Fengning Xiang

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作者单位：

The Key Laboratory of Plant Development and Environmental Adaptation Biology,Ministry of Education,School of Life Sciences,Shandong University,Qingdao 266237,China

State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出版年：

2024

DOI：

10.1111/jipb.13709

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(8)