α1-COP modulates plasmodesmata function through sphingolipid enzyme regulation∞

扫码查看

原文链接

万方数据

外文摘要：Callose,a β-1,3-glucan plant cell wall polymer,reg-ulates symplasmic channel size at plasmodesmata(PD)and plays a crucial role in a variety of plant processes.However,elucidating the molecular mechanism of PD callose homeostasis is limited.We screened and identified an Arabidopsis mutant plant with excessive callose deposition at PD and found that the mutated gene was α1-COP,a member of the coat protein Ⅰ(COPⅠ)coatomer complex.We report that loss of function of α1-COP elevates the callose accumulation at PD by affecting subcellular protein localization of callose degrada-tion enzyme PdBG2.This process is linked to the functions of ERH1,an inositol phosphoryl ceramide synthase,and glucosylceramide synthase through physical interactions with the α1-COP protein.Ad-ditionally,the loss of function of α1-COP alters the subcellular localization of ERH1 and GCS proteins,resulting in a reduction of GlcCers and GlcHCers molecules,which are key sphingolipid(SL)species for lipid raft formation.Our findings suggest that α1-COP protein,together with SL modifiers controlling lipid raft compositions,regulates the subcellular localization of GPI-anchored PDBG2 proteins,and hence the callose tumover at PD and symplasmic movement of biomolecules.Our findings provide the first key clue to link the COPⅠ-mediated intracellular trafficking pathway to the callose-mediated inter-cellular signaling pathway through PD.

外文关键词：

callosecoatomer proteinsmembrane-bound vesicleplasmodesmatasphingolipid enzymes

作者：

Arya Bagus Boedi Iswanto、Minh Huy Vu、Jong Cheol Shon、Ritesh Kumar、Shuwei Wu、Hobin Kang、Da-Ran Kim、Geon Hui Son、Woe Yoen Kim、Youn-Sig Kwak、Kwang Hyeon Liu、Sang Hee Kim、Jae-Yean Kim

展开 >

作者单位：

Division of Applied Life Science(BK21 Four Program),Plant Molecular Biology and Biotechnology Research Center,Gyeongsang National University,Jinju 52828,Korea

College of Pharmacy and Research Institute of Pharmaceutical Sciences,Kyungpook National University,Daegu 702-701,Korea

Division of Applied Life Science(BK21 FOUR Program),Plant Molecular Biology and Biotechnology Research Center,Gyeongsang National University,Jinju 52828,Korea

Department of Agronomy and Plant Genetics,University of Minnesota,St.Paul,USA

Departement of Plant Medicine,Gyeongsang National University,Jinju 52828,Korea

Division of Life Science,Gyeongsang National University,Jinju 52828,Korea

展开 >

出版年：

2024

DOI：

10.1111/jipb.13711

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(8)