首页|PagMYB128 regulates secondary cell wall formation by direct activation of cell wall biosynthetic genes during wood formation in poplar

PagMYB128 regulates secondary cell wall formation by direct activation of cell wall biosynthetic genes during wood formation in poplar

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The biosynthesis of cellulose,lignin,and hemi-celluloses in plant secondary cell walls(SCWs)is regulated by a hierarchical transcriptional regulatory network.This network features or-thologous transcription factors shared between poplar and Arabidopsis,highlighting a founda-tional similarity in their genetic regulation.However,knowledge on the discrepant behavior of the transcriptional-level molecular regulatory mechanisms between poplar and Arabidopsis remains limited.In this study,we investigated the function of PagMYB128 during wood for-mation and found it had broader impacts on SCW formation compared to its Arabidopsis ortholog,AtMYB103.Transgenic poplar trees overexpressing PagMYB128 exhibited sig-nificantly enhanced xylem development,with fiber cells and vessels displaying thicker walls,and an increase in the levels of cellulose,lignin,and hemicelluloses in the wood.In contrast,plants with dominant repression of PagMYB128 demonstrated the opposite phenotypes.RNA sequencing and reverse transcription-quantitative polymerase chain reaction showed that PagMYB1 28 could ac-tivate SCW biosynthetic gene expression,and chromatin immunoprecipitation along with yeast one-hybrid,and effector-reporter assays showed this regulation was direct.Further analysis revealed that PagSND1(SECONDARY WALL-ASSOCIATED NAC-DOMAIN PROTEIN1)directly regulates PagMYB128 but not cell wall metabolic genes,highlighting the pivotal role of PagMYB1 28 in the SND1-driven regulatory network for wood devel-opment,thereby creating a feedforward loop in SCW biosynthesis.

cellulosehemicelluloseligninPagMYB1 28Populustranscription factorwood formation

Yuanyuan Hao、Fachuang Lu、Seung-Won Pyo、Min-Ha Kim、Jae-Heung Ko、Xiaojing Yan、John Ralph、Quanzi Li

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State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing,100091,China

Department of Biochemistry and DOE Great Lakes Bioenergy Research Center,Wisconsin Energy Institute,University of Wisconsin-Madison,Wisconsin 53726,USA

Department of Plant & Environmental New Resources,Kyung Hee University,Yongin 17104,Korea

2024

10.1111/jipb.13717

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(8)