Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet∞

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外文摘要：The ancient crop broomcom millet(Panicum miliaceum L.)is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance.These ad-vantages make it an important alternative crop to increase food security and achieve the goal of zero hunger,particularly in light of the uncertainty of global climate change.However,functional ge-nomic and biotechnological research in broom-com millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques.Here,we successfully per-formed genome editing of broomcorn millet.We identified an elite variety,Hongmi,that produces embryogenic callus and has high shoot re-generation ability in in vitro culture.We established an Agrobacterium tumefaciens-mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9-mediated genome-editing system for Hongmi.Using these techniques,we produced herbicide-resistant transgenic plants and edited phytoene desaturase(PmPDS),which is involved in chlorophyll biosynthesis.To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research,we assembled a near-complete genome sequence of Hongmi and compre-hensively annotated its genome.Together,our re-sults open the door to improving broomcom millet using biotechnology.

外文关键词：

broomcorn milletCRISPR/Cas9 editinggenome assemblyherbicide resistancetransformation system

作者：

Yang Liu、Zixiang Cheng、Weiyao Chen、Chuanyin Wu、Jinfeng Chen、Yi Sui

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作者单位：

State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,the Chinese Academy of Sciences,Beijing 100101,China

State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

University of Chinese Academy of Sciences,Beijing 100049,China

出版年：

2024

DOI：

10.1111/jipb.13664

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(8)