DNA methylation controlling abscisic acid catabolism responds to light to mediate strawberry fruit ripening∞

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外文摘要：Phytohormones,epigenetic regulation and envi-ronmental factors regulate fruit ripening but their interplay during strawberry fruit ripening remains to be determined.In this study,bagged straw-berry fruit exhibited delayed ripening compared with fruit grown in normal light,correlating with reduced abscisic acid(ABA)accumulation.Transcription of the key ABA catabolism gene,ABA 8'-hydroxylase FaCYP707A4,was induced in bagged fruit.With light exclusion whole genome DNA methylation levels were up-regulated,cor-responding to a delayed ripening process,while DNA methylation levels in the promoter of FaCYP707A4 were suppressed,correlating with increases in transcript and decreased ABA con-tent.Experiments indicated FaCRY1,a blue light receptor repressed in bagged fruit and FaAGO4,a key protein involved in RNA-directed DNA methylation,could bind to the promoter of FaCYP707A4.The interaction between FaCRY1 and FaAGO4,and an increased enrichment of FaAGO4 directed to the FaCYP707A4 promoter in fruit grown under light suggests FaCRY1 may influence FaAGO4 to modulate the DNA methyl-ation status of the FaCYP707A4 promoter.Fur-thermore,transient overexpression of FaCRY1,or an increase in FaCRY1 transcription by blue light treatment,increases the methylation level of the FaCYP707A4 promoter,while transient RNA in-terference of FaCRY1 displayed opposite phe-notypes.These findings reveal a mechanism by which DNA methylation influences ABA catabo-lism,and participates in light-mediated straw-berry ripening.

外文关键词：

ABA catabolismDNA methylationlightripeningstrawberry

作者：

Yunfan Sun、Xiaofang Yang、Rongrong Wu、Shouzheng Lv、Yunduan Li、Haoran Jia、Yuying Yang、Baijun Li、Wenbo Chen、Andrew C.Allan、Guihua Jiang、Yan-Na Shi、Kunsong Chen

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作者单位：

College of Agriculture & Biotechnology,Zhejiang University,Zijingang Campus,Hangzhou 310058,China

Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China

Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Zijingang Campus,Hangzhou 310058,China

The State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Zijingang Campus,Hangzhou 310058,China

New Zealand Institute for Plant & Food Research Limited,Private Bag 92169,Auckland,New Zealand

School of Biological Sciences,University of Auckland,Private Bag 92019,Auckland,New Zealand

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出版年：

2024

DOI：

10.1111/jipb.13681

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(8)